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| Metabolic Engineering of Candida tropicalis for Xyltiol Production from Xylose Mother Liquor |
| CHEN Zhen1,2, CHEN Xian-zhong1,2, ZHANG Li-hua1,2, WANG Jun-hua3, SHEN Wei1,2, FAN You1,2 |
1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;
2. Center of Bioresource & Bioenergy, School of Biotechnology, Jiangnan University, Wuxi 214122, China;
3. National Engineering Laboratory for Creal Fermention Technology, Jiangnan University, Wuxi 214122, China |
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Abstract As one of polyol, xylitol has been extensively used in the food, pharmaceutical and other light industries. Presently, xylitol is produced by the reduction of D-xylose derived from hemicellulosic hydrolysate in the presence of Raney nickel catalysts. In the process of extracting xylose, a large amount of xylose mother liquor as by-product will be generated. Xylose mother liquor contains in a certain concentration of glucose, xylose, arabinose and other carbon sources, and a small amount of furfural, tetrahydrofuran and other substances. Production of biochemical from xylose mother liquor via microbial transformation can improve economic value of xylose mother liquor and reduce the environmental pollution. Candida tropicalis can not only use glucose, but also has a highly efficient xylose metabolic pathway. Two allelic genes of xylitol dehydrogenase gene from C. tropicalis was deleted by genetic technique, and the resulting mutant strain was obtained, which can not use xylitol as carbon source. The fermentation performance of the mutant in xylose mother liquor medium was evaluated. The fermentation conditions of xylitol fermentation process was optimized. The optimal fermentation conditions were as follows:300g/L xylose mother liquor, 5g/L corn steep liquor, fermentation temperature 35℃, initial pH 5.0, inoculate volume 15%, under 200r/min for 140h. Using the optimized fermentation process, xylitol yield reached 83.01g/L.
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Received: 11 November 2016
Published: 25 May 2017
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