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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (9): 117-123    DOI:
    
Research Process on Microbial Diversity of Producing-nitrile Hydratase and Study on Nitrile Hydratase
WANG Shi-wei1,2, WANG Min1
1. Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science &Technology, Ministry of Education, Tianjin 300457, China;
2. College of Life Sciences and Engineering of Qiqihar University, Qiqihar 161006, China
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Abstract  

The reactions catalyzed by nitrile hydratase have been widely applied in organic synthesis. As an important class of catalytic agent, NHase (nitrile hydratase EC4.2.1.84) can converts nitriles to the corresponding acidamide. Because of their inherent enantio-and regioselectivities and other benefits, NHases are attractive as green, mild, and selective catalysts for setting stereogenic centers in fine-chemical synthesis and enantiospecific synthesis of a variety of acrylamides. The literature has been surveyed to provide a comprehensive coverage of the study of NHases in China at present including biodiversity of strain-producing NHase, biochemical and structural properties of NHase, strain improvement, as well as construction of gene engineering strains. Literature has also been cited to describe methods of immobilized cells and application of nitrile hydratase.



Key wordsMicrobial diversity      Nitrile hydratase      Research process     
Received: 20 May 2011      Published: 25 September 2011
ZTFLH:  Q55  
Cite this article:

WANG Shi-wei, WANG Min. Research Process on Microbial Diversity of Producing-nitrile Hydratase and Study on Nitrile Hydratase. China Biotechnology, 2011, 31(9): 117-123.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I9/117


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