Production and Copper Acquisition Ability of Methanobactin

China Biotechnology

2011, Vol. 31

Issue (8): 40-46 DOI:

Production and Copper Acquisition Ability of Methanobactin

XIN Jia-ying^1,2, DONG Jing¹, YAN Chao-zhe¹, QIAO Jun¹, LIANG Hong-ye¹, XIA Chun-gu²

1. Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150076,China;
2. State Key Laboratory for Oxo Synthesis and Selective Oxidation,Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou 730000,China

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Abstract

Methanobactin(mb)is biochelator that appears to function as an agent for copper acquisition and uptake in methanotrophs. The production of mb during growth of Methylosinus trichosporium IMV 3011 on methane and methanol was determined by split nitrate mineral salts/CAS-Cu(chrome azurol S)plates. mb was found to be accumulated in the spent medium of Methylosinus trichosporium IMV 3011 under copper-limited condition. The mb production ability of methanol-grown cells were obviously higher than that of methane-grown cells. It has been speculated that methanol can act as the electron-donating substrate to regenerate the NADH and drive mb synthesis. The effect of mb from Methylosinus trichosporium IMV3011 on the growth lag,growth rate and whole-cell pMMO(particles Methane Monooxygenase)activity of other methanotrophs were measured respectively to assess the specifity of mb. It has been shown that mb from Methylosinus trichosporium IMV 3011 can accelerate the expression of whole cell pMMO activity of other methanotrophs when cultures are exposed to elevatory copper concentration. Also,mb from Methylosinus trichosporium IMV 3011 can shorten the growth lag and increase the growth rate of other methanotrophs either in elevatory or constant copper concentration. The results suggested that other methanotroph may take delivery of copper from mb released by Methylosinus trichosporium IMV 3011.

Key words： Methanobactin Copper acquisition Split plates Particles methane monooxygenase Methanotrophs

Received: 14 March 2011 Published: 25 August 2011

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XIN Jia-ying, DONG Jing, YAN Chao-zhe, QIAO Jun, LIANG Hong-ye, XIA Chun-gu. Production and Copper Acquisition Ability of Methanobactin. China Biotechnology, 2011, 31(8): 40-46.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I8/40

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