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| Surface Display Expression of Helicobacter pylori Ure B Fusion Epitope |
| FENG Xiao-yan1,2, WANG Yang-chun2, LI Ping-chao2, TAO Hao-xia2, WANG Peng2, YUAN Sheng-ling2, WANG Ling-chun2, WANG Pu1, LIU Chun-jie2 |
1. College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310032,China;
2. Beijing Institute of Biotechnology,State Key Laboratory of Pathogen and Biosecurity,Beijing 100071,China |
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Abstract Objective:To achieve surface display of H.pylori ure B fusion epitope by vector pHIE3N. Methods:Multiple cloning site was introduced into pHIE3N in order to express the H.pylori ure B fusion epitope protein. The recombinant strain was confirmed by SDS-PAGE,Western blot and ELISA. Result:The inserted sequence of the reconstructed plasmid was correct. The molecular weight of the fused Ure B protein was about 60 kDa which had proved by SDS-PAGE and Western blot analysis,ELISA results showed that the Ure B fusion epitope expressed on cell surfaces. Conclusion:The Ure B fusion epitope was successfully expressed on the sruface of E.coli DH5α using pHIE3N surface display vector. This result will contribute to the vaccine research of H.pylori.
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Received: 22 March 2011
Published: 25 August 2011
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Cite this article:
FENG Xiao-yan, WANG Yang-chun, LI Ping-chao, TAO Hao-xia, WANG Peng, YUAN Sheng-ling, WANG Ling-chun, WANG Pu, LIU Chun-jie. Surface Display Expression of Helicobacter pylori Ure B Fusion Epitope. China Biotechnology, 2011, 31(8): 35-39.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I8/35
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