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| The Determination and Control of Coproporphyrin Ⅲ in Vitamin B12 Fermentation by Pseudomonas denitrificans |
| WANG Ze-jian, ZHAO Lin-lin, CHU Ju, ZHANG Ying-ping, ZHANG Si-liang |
| State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China |
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Abstract The accumulation of coproporphyrin Ⅲ,which is byproduct generated in vitamin B12 fermentation of Pseudomonas denitrificans,greatly affect the biosynthesis of vitamin B12 and the extraction in industrial production. An effective method for determining coproporphyrin Ⅲ concentration directly after suitable treatment of the fermentation broth. Furthermore,the influence of oxygen supply levels,carbon dioxide concentrations and pH on coproporphyrin Ⅲ biosynthesis were investigated,the optimal control strategy was implemented in 120 m3 industrial bioreactor. The results revealed that higher oxygen supply promoted the coproporphyrin Ⅲ accumulation,controlling the inlet carbon dioxide concentration at 8.6±0.8% and pH at 7.0±0.12 could greatly inhibit the coproporphyrin Ⅲ biosynthesis,and the vitamin B12 production increased for 15% than that of control.
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Received: 31 March 2011
Published: 25 August 2011
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