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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (06): 58-63    DOI:
    
Bioactivity Screening of Fermentation Crude Extracts from Deep Sea Metagenomic Library Clones
TANG Xi-xiang1,2, YI Zhi-wei2, LI Ning1, MA Qun1, LI Hui1, QIN Dan2, XIAO Xiang1
1. School of Life Science, Xiamen University, Xiamen 361005, China;
2. Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
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Abstract  

Objective: Detect bioactivities of fermentation crude extracts originated from previously constructed deep sea metagenomic libraries. Methods: Functional screening of antitumor(cytotoxic),antibacterial and anti HBV activity from fermentation crude extracts was performed by CCK-8 method, agar diffusion method, double agar method and real time PCR method. Results: No antibacterial and anti HBV clones were identified and three mixed clones showed good cytotoxic activity. HPLC analysis of these three mixed clones fingerprints indicated they possess obviously different fingerprints with E. coli host which could be useful to next isolation of cytotoxic compounds from deep sea metagenomic library.Conclusion: These results indicated that chemical screening could be potential way for screening bioactivity substance from deep sea metagenomic libraries besides functional screening and homology screening.



Key wordsDeep sea      Metagenomic      Bioactivity screening      HPLC fingerprints     
Received: 01 August 2010      Published: 28 June 2011
ZTFLH:  Q93  
Corresponding Authors: TANG Xi-Xiang     E-mail: tangxixiang@hotmail.com
Cite this article:

TANG Xi-xiang, YI Zhi-wei, LI Ning, MA Qun, LI Hui, QIN Dan, XIAO Xiang. Bioactivity Screening of Fermentation Crude Extracts from Deep Sea Metagenomic Library Clones. China Biotechnology, 2011, 31(06): 58-63.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I06/58

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