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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2023, Vol. 43 Issue (9): 105-112    DOI: 10.13523/j.cb.2303011
综述     
芳香族氨基酸脱羧酶研究进展*
刘晴浩1,李陈曦1,包心茹1,祁峰1,2,**()
1 福建师范大学生命科学学院 工业微生物发酵技术国家地方联合工程研究中心 福州 350117
2 2 福建师范大学细胞逆境响应与代谢调控福建省高校重点实验室 福州 350108
Advances in Aromatic L-amino Acid Decarboxylases
LIU Qing-hao1,LI Chen-xi1,BAO Xin-ru1,QI Feng1,2,**()
1 National and Local Joint Engineering Research Center for Industrial Microbial Fermentation Technology, College of Life Science, Fujian Normal University, Fuzhou 350117, China
2 Fujian Provincial Key Laboratory of Cell Stress Response and Metabolic Regulation, Fujian Normal University, Fuzhou 350108, China
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摘要：

芳香族氨基酸脱羧酶(aromatic L-amino acid decarboxylases,AADCs)在生物体内的作用是将芳香族氨基酸脱羧转化为芳香族单胺(aromatic monoamines),磷酸吡哆醛(pyridoxal 5'-phosphate,PLP)是其行使催化功能时必不可少的辅酶。AADCs催化芳香族氨基酸产生的芳香族单胺,主要包括多巴胺、血清素、酪胺、色胺等,这些芳香族单胺在生物体内是维持正常生理功能的神经递质,也是参与合成某些化合物的重要前体,还可作为药物中的活性成分参与治疗多种人类疾病,具有广阔的应用前景。作为生物合成芳香族单胺所必需的酶,有关AADCs的研究也越来越深入,基于AADCs的芳香族单胺生物合成也取得了长足进步。对几种主要AADCs进行综述,为AADCs更好应用于芳香族单胺的生物合成提供参考。
关键词： 芳香族氨基酸脱羧酶;  芳香族单胺;  蛋白质结构;  定向改造    
Abstract:

Aromatic L-amino acid decarboxylase (AADC)’s role in living organisms is to decarboxylate aromatic L-amino acids into aromatic monoamines, and pyridoxal 5'-phosphate (PLP) is an essential coenzyme for its catalytic function. AADCs transform aromatic L-amino acids to aromatic monoamines, mainly including dopamine, serotonin, tyramine, and tryptamine. These aromatic monoamines are neurotransmitters that maintain normal physiological functions in living organisms and are also important precursors involved in the synthesis of some compounds. Futhermore, they can also be used as active ingredients in drugs to participate in the treatment of many human diseases, with promising applications. As the enzymes necessary for the biosynthesis of aromatic monoamines, AADCs have attracted more researches’ attention, and great progress has also been made in the biosynthesis of aromatic monoamines based on AADCs. Here several major AADCs are reviewed to provide references for better applications of AADCs in the biosynthesis of aromatic monoamines.
Key words: Aromatic L-amino acid decarboxylase    Aromatic monoamines    Protein structure    Directed remodelling
收稿日期: 2023-03-04 出版日期: 2023-10-08
ZTFLH:  Q814  
基金资助: * 国家自然科学基金(32272287);国家现代农业(糖料)产业技术体系建设专项(CARS-170501)
通讯作者: ** 电子信箱:f.qi@fjnu.edu.cn   
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引用本文:

刘晴浩, 李陈曦, 包心茹, 祁峰. 芳香族氨基酸脱羧酶研究进展*[J]. 中国生物工程杂志, 2023, 43(9): 105-112.

LIU Qing-hao, LI Chen-xi, BAO Xin-ru, QI Feng. Advances in Aromatic L-amino Acid Decarboxylases. China Biotechnology, 2023, 43(9): 105-112.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2303011    或    https://manu60.magtech.com.cn/biotech/CN/Y2023/V43/I9/105

图1  不同AADCs催化的反应
图2  CrTDC与PLP的结合
图3  CrTDC催化底物脱羧的反应机制
图4  ADCs系统进化树分析
图5  AADCs氨基酸序列与蛋白质结构的比较
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