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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2021, Vol. 41 Issue (4): 74-80    DOI: 10.13523/j.cb.2012028
综述     
黄素依赖型卤化酶的结构特点及工程改造*
王一涵,李海岩,薛永常()
大连工业大学生物工程学院 大连 116034
The Structural Characteristics and Engineering Reconstruction of Flavin-dependent Halogenase
WANG Yi-han,LI Hai-yan,XUE Yong-chang()
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China
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摘要：

卤化物是通过卤化酶催化卤族元素在有机化合物上特定位置发生取代形成的一类化合物,具有独特的生理生化作用。黄素依赖型卤化酶具有良好的区域选择性,虽然有相似的黄素分子的结合位点,但在底物结合方面略有不同,对其结构和合成途径及结合蛋白质工程的随机诱变和定向改造的研究在工业应用中至关重要。讨论了具有高区域选择性的黄素依赖型卤化酶的结构特点及工程改造,以及经过工程改造后黄素依赖型卤化酶在工业生产中的应用。
关键词： 黄素依赖型卤化酶;  高选择性;  结构特点;  定向改造    
Abstract:

Halides are a kind of compounds which catalyzed by halogenase to the addition of halogen in secondary metabolites, have unique physiological and biochemical functions. Flavin-dependent halogenases have good regional selectivity and stability, and similar FAD binding sites with slight difference in substrates made them very important in industrial application. Therefore, the studies on its structure and synthetic pathway as well as random mutagenesis and directed modification of protein engineering are very important for its industry application. In this paper, the structural characteristics and engineering modification of flavin-dependent halogenases with high regional selectivity are reviewed, and it is of guiding significance for the application of engineering-modification flavin-dependent halides in industrial production.
Key words: Flavin-dependent halogenase    High selectivity    Structural characteristics    Directional transformation
收稿日期: 2020-12-17 出版日期: 2021-04-30
ZTFLH:  Q819  
基金资助: *辽宁省自然科学基金资助项目(20180550858)
通讯作者: 薛永常     E-mail: xueych@dlpu.edu.cn
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引用本文:

王一涵,李海岩,薛永常. 黄素依赖型卤化酶的结构特点及工程改造*[J]. 中国生物工程杂志, 2021, 41(4): 74-80.

WANG Yi-han,LI Hai-yan,XUE Yong-chang. The Structural Characteristics and Engineering Reconstruction of Flavin-dependent Halogenase. China Biotechnology, 2021, 41(4): 74-80.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2012028    或    https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I4/74

图1  PrnA和PyrH的晶体结构[32]
图2  VirX1的蛋白质结构
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