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重组白蛋白结合肽融合人白介素-11的原核可溶表达、纯化、表征及初步活性评价* |
孟奂,粟乙梵,王洒,张淑雯,郑永祥,余蓉,张纯**() |
四川大学华西药学院 靶向药物及释药系统教育部重点实验室 成都 610041 |
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Prokaryotic Soluble Expression, Purification, Characterization and Preliminary Activity Evaluation of Recombinant Albumin Binding Peptide Fused with Human Interleukin-11 |
Huan MENG,Yi-fan SU,Sa WANG,Shu-wen ZHANG,Yong-xiang ZHENG,Rong YU,Chun ZHANG**() |
Key Laboratory of Drug Targeting and Drug Delivery System (Ministry of Education), West China School of Pharmacy, Sichuan University, Chengdu 610041, China |
引用本文:
孟奂, 粟乙梵, 王洒, 张淑雯, 郑永祥, 余蓉, 张纯. 重组白蛋白结合肽融合人白介素-11的原核可溶表达、纯化、表征及初步活性评价*[J]. 中国生物工程杂志, 2023, 43(7): 44-52.
Huan MENG, Yi-fan SU, Sa WANG, Shu-wen ZHANG, Yong-xiang ZHENG, Rong YU, Chun ZHANG. Prokaryotic Soluble Expression, Purification, Characterization and Preliminary Activity Evaluation of Recombinant Albumin Binding Peptide Fused with Human Interleukin-11. China Biotechnology, 2023, 43(7): 44-52.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2212024
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https://manu60.magtech.com.cn/biotech/CN/Y2023/V43/I7/44
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[1] |
Dorner A J, Goldman S J, Keith J C. Interleukin-11. BioDrugs, 1997, 8(6): 418-429.
pmid: 18031104
|
[2] |
Karpovich N, Chobotova K, Carver J, et al. Expression and function of interleukin‐11 and its receptor α in the human endometrium. Molecular Human Reproduction, 2003, 9(2): 75-80.
pmid: 12569176
|
[3] |
Liu Z R, Wang Y X, Yan J X, et al. Efficacy and safety of recombinant human interleukin-11 in the treatment of acute leukaemia patients with chemotherapy-induced thrombocytopenia: a systematic review and meta-analysis. Journal of Evaluation in Clinical Practice, 2020, 26(1): 262-271.
doi: 10.1111/jep.13152
pmid: 31222870
|
[4] |
Cook S A, Schafer S. Hiding in plain sight: interleukin-11 emerges as a master regulator of fibrosis, tissue integrity, and stromal inflammation. Annual Review of Medicine, 2020, 71: 263-276.
doi: 10.1146/annurev-med-041818-011649
pmid: 31986085
|
[5] |
Ng B, Dong J R, D’Agostino G, et al. Interleukin-11 is a therapeutic target in idiopathic pulmonary fibrosis. Science Translational Medicine, 2019, 11(511): eaaw1237.
doi: 10.1126/scitranslmed.aaw1237
|
[6] |
Allanki S, Strilic B, Scheinberger L, et al. Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration. Science Advances, 2021, 7(37): eabg6497.
doi: 10.1126/sciadv.abg6497
|
[7] |
唐佳, 许晓玲, 聂小霞, 等. 重组人IL-11的原核表达、纯化和鉴定. 生物医学工程学杂志, 2012, 29(3): 530-533.
pmid: 22826953
|
|
Tang J, Xu X L, Nie X X, et al. Prokaryotic expression, purification, and ide.pngication of recombinant human IL-11 protein. Journal of Biomedical Engineering, 2012, 29(3): 530-533.
pmid: 22826953
|
[8] |
McCoy J, Diblasio-Smith E, Grant K, et al. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules: US, US5646016A. 1997-07-08[2022-12-18]. https://kns.cnki.net/kcms2/article/abstract?v=kxaUMs6x7-6tfk4cqdLU9Xr6rnIkrlu2bfJW2lhc9_JGbELygDmHfEH1vjSyVDxeb4T8qrPsNsXgUoBTt19giRU8yNaVxuMe&uniplatform=NZKPT.
|
[9] |
Tan H D, Dan G P, Gong H Y, et al. Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli. Biotechnology Letters, 2005, 27(13): 905-910.
doi: 10.1007/s10529-005-7179-3
|
[10] |
张颖, 刘灿辉, 刘玉乐, 等. 人白细胞介素11cDNA的克隆、融合表达及活性测定. 生物工程学报, 2000, 16(3): 353-356.
pmid: 11059280
|
|
Zhang Y, Liu C H, Liu Y L, et al. cDNA cloning, fusion expression in Escherichia coli and activity assay of hIL-11. Chinese Journal of Biotechnology, 2000, 16(3): 353-356.
pmid: 11059280
|
[11] |
Nguyen T Q, Duong H, Dang T N H, et al. Enhanced soluble expression and effective purification of recombinant human interleukin-11 by SUMO fusion in Escherichia coli. Indian Journal of Biotechnology, 2018, 17(4): 579-585.
|
[12] |
Lee H T, Park S W, Kim M, et al. Interleukin-11 protects against renal ischemia and reperfusion injury. American Journal of Physiology-renal Physiology, 2012, 303(8): F1216-F1224.
doi: 10.1152/ajprenal.00220.2012
|
[13] |
Nilvebrant J, Hober S. The albumin-binding domain as a scaffold for protein engineering. Computational and Structural Biotechnology Journal, 2013, 6(7): e201303009.
|
[14] |
Johansson M U, Frick I M, Nilsson H, et al. Structure, specificity, and mode of interaction for bacterial albumin-binding modules. The Journal of Biological Chemistry, 2002, 277(10): 8114-8120.
doi: 10.1074/jbc.M109943200
|
[15] |
Hsu F T, Liu Y C, Tsai C L, et al. Preclinical evaluation of recombinant human IL15 protein fused with albumin binding domain on anti-PD-L1 immunotherapy efficiency and anti-tumor immunity in colon cancer and melanoma. Cancers, 2021, 13(8): 1789.
doi: 10.3390/cancers13081789
|
[16] |
Jonsson A, Dogan J, Herne N, et al. Engineering of a femtomolar affinity binding protein to human serum albumin. Protein Engineering, Design and Selection, 2008, 21(8): 515-527.
doi: 10.1093/protein/gzn028
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