蛋白质结晶是近年来生物化学领域的研究热点。X射线衍射法是可以获得蛋白质三维结构的唯一可靠途径，但前提条件是要得到合适的蛋白质晶体，如何获得蛋白质晶体是对蛋白质结构分析的瓶颈。综述了近几十年蛋白质结晶的研究进展，重点介绍了蛋白质结晶的原理以及蛋白质结晶的四种常用方法：蒸发扩散结晶法；批量结晶法; 透析结晶法; 液液扩散结晶法，以及结晶策略，并展望了今后的研究前景。
Protein crystallization is the hot research area in biochemistry and X-ray diffraction is the most reliable method to determine the three-dimensional structure of proteins, but it can only be applied provided suitable crystals are obtained. Obtaining good ordered crystals is currently the major barrier to structure determination. This paper present recent investigation in protein crystallization and emphasize in four major techniques for growing crystals of biological macromolecules: liquid/liquid diffusion ，batch crystallization, vapor diffusion and dialysis, and also mention to detergents and strategy for protein crystallization.
葛巍巍,任卓英,戴晓畅. 蛋白质结晶方法的研究进展[J]. 中国生物工程杂志, 2006, 26(0): 208-211.
. Recent advances in methodology for the crystallization of protein. China Biotechnology, 2006, 26(0): 208-211.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/208
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