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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (6): 77-85    DOI: 10.13523/j.cb.20180611
综述     
铁蛋白的生物工程应用 *
王玲,吴洋,张盛,齐浩()
天津大学化工学院 系统生物工程教育部重点实验室 天津大学化工协同创新中心合成生物学平台 天津 300072
Bioengineering Application of Ferritin
Ling WANG,Yang WU,Sheng ZHANG,Hao QI()
Key Laboratory of Systems Bioengineering of Ministry of Education, SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China
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摘要：

铁蛋白是一种普遍存在于生物体内的储铁蛋白,具有铁离子代谢、抗氧化胁迫及消除其他过量金属离子毒害作用的功能。随着对铁蛋白结构和生化功能认识的深入,铁蛋白作为一个含有四氧化三铁核心的特殊蛋白复合体,被广泛应用于生物医学、纳米材料、生物分子成像等各种生物工程领域。该综述针对已知的主要铁蛋白分子,论述了铁蛋白的结构及酶活性机理,基于铁蛋白的多功能分子骨架应用,以及基于铁蛋白磁性的生物分子开关等热点研究,最后对铁蛋白生物工程、生物医学领域的应用和发展进行了展望。
关键词： 铁蛋白;  磁性颗粒;  分子骨架    
Abstract:

Widely conserved iron-storage protein plays crucial role in ferric ion metabolism maintaining iron homeostasis, resisting oxidative stress and eliminating other toxic effects of excessive metal ions. With gaining the knowledge from structural and molecular studies, iron-storage protein complex in which iron cluster formed from absorbing free iron has been successfully applied in fields of biomedical engineering, nanomaterial, and biomolecule imaging. Herein, the recent progresses in studying the catalytic mechanism of iron cluster formation are briefly introduced. And the cutting-edge bioengineering applications in which iron-storage protein were engineered as a versatile molecular scaffold for presenting special chemicals, or natural magnetic particle for constructing remote control molecular machine are reviewed, although related researches has arisen academic argument.
Key words: Ferritin    Magnetic particle    Molecular scaffold
收稿日期: 2018-01-29 出版日期: 2018-07-06
ZTFLH:  Q71  
基金资助: * 国家自然科学基金资助项目(21476167)
通讯作者: 齐浩     E-mail: haoq@tju.edu.cn
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引用本文:

王玲,吴洋,张盛,齐浩. 铁蛋白的生物工程应用 *[J]. 中国生物工程杂志, 2018, 38(6): 77-85.

Ling WANG,Yang WU,Sheng ZHANG,Hao QI. Bioengineering Application of Ferritin. China Biotechnology, 2018, 38(6): 77-85.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180611    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I6/77

图1  铁蛋白及铁蛋白亚基结构示意图
图2  铁蛋白铁氧化酶活性中心作用机制示意图
图3  铁蛋白功能的人工改造示意图
图4  铁蛋白的修饰方法
图5  铁蛋白磁性控制开关
图6  铁蛋白解离与组装的条件转变
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