在链霉素对杀虫抗生素菌株GX-29孢子的致死浓度测定基础上，采用紫外和微波诱变相结合的方法对菌株孢子进行诱变处理，诱变处理的孢子涂布在含链霉素最小抑制浓度（18.2μg/mL）的高氏平板上，获得了大量的链霉素抗性基因(sty)突变株。进一步通过摇瓶复筛，获得高产突变株L8，其摇瓶发酵的杀虫活性为出发菌株1.94倍。该菌株经多次传代，遗传性状较稳定。
After testing the resistance 0f streptomycin to GX-29，the spores treated with UV light and microwave were regenerated on agar plants containing 18.2μg/mL streptomycin. A number of streptomycin resistant (str) mutants were obtained. Then we acquired a high antibiotic-producing strain L8 through re-screening by batch fermentation.The activity of the mutant L8 increased by 1.94 times over the original strain．The high productivity could be inherited after several subcultures.
凌成金,张云开,邵利,覃拥灵,梁智群. 杀虫抗生素高产菌株的选育研究[J]. 中国生物工程杂志, 2008, 28(专刊): 120-123.
. A STUDY ON THE BREEDING OF HIGH INSECTICIDAL ANTIBIOTICS STRAIN. China Biotechnology, 2008, 28(专刊): 120-123.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I专刊/120
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