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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2017, Vol. 37 Issue (9): 98-104    DOI: 10.13523/j.cb.20170913
技术与方法     
亚麻芥种子油体的提取及稳定性研究
高红桃, 郭晓威, 孙丹, 解长睿, 王法微, 李海燕
吉林农业大学生物反应器与药物开发教育部工程研究中心/生命科学学院 长春 130118
Extraction of Camelina Seed Oil Body and Analysis of Stability
GAO Hong-tao, GUO Xiao-wei, SUN Dan, XIE Chang-rui, WANG Fa-wei, LI Hai-yan
1. College of Life Sciences, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China
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摘要： 油体是植物种子内储藏脂肪的亚细胞单位，其内部为三酰甘油（TAG），外部由单层磷脂和油体蛋白组成。这种稳定的结构可以使油体抵抗环境的压力，便于应用到食品及制药工业中。对比碱法、水法、Tris-HCl法、Tricine法4种油体提取方法，对亚麻芥油体采取梯度离心法，制备亚麻芥油体，并对不同pH、温度、NaCl浓度条件下油体稳定性做初步研究。结果表明，4种方法所提取的油体大小均在0.5~1μm；相较于水法、Tricine法、碱法所回收油体被破坏，Tris-HCl法提取油体大小均一，提取率达17%。同时，油体在4 ≤ pH ≤ 7条件下，油体稳定性破坏。8 ≤ pH ≤ 10时；油体均匀分散，稳定分布。经过不同温度及NaCl浓度处理的油体均呈上浮状态，稳定性破坏。
关键词： 油体蛋白;  粒径;  油体;  稳定性;  显微结构    
Abstract: Oil bodies are specialized organelles found regularly in plant oil seeds which save as lipid storage compartment. Triacylglycerols (TAG) are present in these small discrete intracellular organelles and oil bodies was surrounded by a monolayer phospholipids embedded with some specific proteins. The oil body can be applied to the food and pharmaceutical industry by the stable structure of against stress environment. Extraction efficiency camelina oil body was compared under the different method and stability was investigated including that the different pH, NaCl concentration conditions of camelina oil.The rate of extracted for oil bodies was up to 17% and uniform of dispersion be proved. The stability of oil bodies decreased with the increase of NaCl concentration; the oil bodies stably distributed in 8 ≤ pH ≤ 10 and the oil bodies' stability decreased obviously when pH value was between 4 and 7.The temperature and NaCl concentration almost had no effects on the oil bodies' stability.
Key words: Oil bodies    Oleosin    Structure of microscope    Size    Stability
收稿日期: 2017-01-20 出版日期: 2017-09-25
ZTFLH:  Q819  
基金资助: 国家“863”计划资助项目（2011AA1000691008）
通讯作者: 王法微, 李海燕     E-mail: fw-1980@163.com;hyli99@163.com
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孙丹
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引用本文:

高红桃, 郭晓威, 孙丹, 解长睿, 王法微, 李海燕. 亚麻芥种子油体的提取及稳定性研究[J]. 中国生物工程杂志, 2017, 37(9): 98-104.

GAO Hong-tao, GUO Xiao-wei, SUN Dan, XIE Chang-rui, WANG Fa-wei, LI Hai-yan. Extraction of Camelina Seed Oil Body and Analysis of Stability. China Biotechnology, 2017, 37(9): 98-104.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20170913    或    https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I9/98

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