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利用CpG DNA甲基化酶M.Sss I共表达载体制备限制性内切酶Not I |
王佩1,2, 陈凯1,2, 高嵩1,2,3 |
1. 淮海工学院江苏省海洋药物活性分子筛选重点实验室 连云港 222005; 2. 淮海工学院江苏省海洋生物产业技术协同创新中心 连云港 222005; 3. 江苏省海洋资源开发研究院 连云港 222005 |
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Production of Restriction Endonuclease Not I Utilizing CpG DNA Methylase M.Sss I Co-expression Vector |
WANG Pei1,2, CHEN Kai1,2, GAO Song1,2,3 |
1. Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China; 2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China; 3. Jiangsu Marine Resources Development Research Institute, Lianyungang 222005, China |
[1] Pingoud A, Wilson G G, Wende W. Type Ⅱ restriction endonucleases——a historical perspective and more. Nucleic Acids Research, 2014, 42(12):7489-7527. [2] Williams R J. Restriction endonucleases:classification, properties, and applications. Molecular Biotechnology,2003,23(3)225-243. [3] 张巧, 叶贤龙, 任桂萍, 等. 限制性内切酶Not I提纯的新工艺. 中国生物工程杂志, 2011, 31(8):102-109. Zhang Q, Ye X L, Ren G P, et al. A new method for the purification of restriction enzyme Not I. China Biotechnology, 2011, 31(8):102-109. [4] Naito T, Kusano K, Kobayashi I. Selfish behavior of restriction-modification systems. Science, 1995, 267(5199):897-899. [5] Smith H O, Nathans D. A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. Journal of Molecular Biology, 1973, 81(3):419-423. [6] Haberman A, Heywood J, Meselson M. DNA modification methylase activity of Escherichia coli restriction endonucleases K and P. Proceedings of the National Academy of Sciences of the United States of America, 1972, 69(11):3138-3141. [7] Lunnen K D, Barsomian J M, Camp R R, et al. Cloning type-Ⅱ restriction and modification genes. Gene, 1988, 74(1):25-32. [8] Zhu Z Y, Quimby A, Guan S X, et al. High fidelity restriction endonucleases. US Patent, US20110151450A1, 2011-6-23. [9] Zhu Z Y, Blanchard A, Xu S X, et al. High fidelity restriction endonucleases. US Patent, US20090029376A1, 2009-1-29. [10] Morgan R D, Camp R R, Wilson G G, et al. Molecular cloning and expression of NlaⅢ restriction-modification system in E. coli. Gene, 1996, 183(1-2):215-218. [11] Renbaum P, Abrahamove D, Fainsod A, et al. Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M SssI). Nucleic Acids Research, 1990, 18(5):1145-1152. [12] Thermo Fisher Scientific Inc. Thermo Scientific Molecular Biology Solutions. Thermo Fisher Scientific Inc., MA, USA, 2012-2013. [13] Richard D M, Middleton, Jack S B, et al. Isolation DNA encoding the Not I restriction endonuclease and relation methods for producing the same. US Patent, 5371006, 1994-12-6. [14] Konstantinovic M, Maksimovic V, Nikcevic G, et al. Hybrid PLtl promoter with dual regulation control. DNA and Cell Biology, 1991, 10(5):389-395. [15] Cheong D E, Choi J H, Song J J, et al. Construction of non-invasively constitutive expression vectors using a metagenome-derived promoter for soluble expression of proteins. Bioprocess and Biosystems Engineering, 2013, 36(6):667-676. [16] Sabido A, Martínez L M, de Anda R, et al. A novel plasmid vector designed for chromosomal gene integration and expression:use for developing a genetically stable Escherichia coli melanin production strain. Plasmid, 2013, 69(1):16-23. [17] Waite-Rees P A, Keating C J, Moran L S, et al. Characterization and expression of the Escherichia coli Mrr restriction system. Journal of Bacteriology, 1991, 173(16):5207-5219. |
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