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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (9): 24-30    DOI: 10.13523/j.cb.20140905
研究报告     
IQGAP1基因干扰对人食管癌细胞同质粘附能力的影响
王康1, 贺春1, 高伟2, 王斌全2, 王晓霞1
1. 山西医科大学生物化学与分子生物学教研室 太原 030001;
2. 山西医科大学第一医院 太原 030001
Effect of IQGAP1 Knockdown on Homotypic Cell-Cell Adhesion of Esophageal Carcinoma Cells
WANG Kang1, HE Chun1, GAO Wei2, WANG Bin-quan2, WANG Xiao-xia1
1. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China;
2. Department of Otolaryngology Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan 030001, China
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摘要：

目的：研究IQGAP1基因干扰对人食管癌细胞同质粘附能力的影响。方法：体外培养人食管癌KYSE150和 EC9706细胞，利用Western blot方法检测两株细胞IQGAP1蛋白的表达，利用缓慢聚集和细胞分离实验比较两株细胞同质粘附能力的差异；进一步在KYSE150和EC9706细胞中构建IQGAP1基因干扰的稳定细胞系，观察IQGAP1基因干扰后细胞同质粘附能力的改变。结果：KYSE150细胞IQGAP1蛋白表达量低于EC9706细胞，而同质粘附能力高于EC9706细胞；IQGAP1基因干扰后，其蛋白表达量明显降低，而细胞同质粘附能力明显增强。结论：IQGAP1 基因干扰能够显著增强食管癌细胞的同质粘附能力，从而降低肿瘤细胞的恶性表型。
关键词： IQGAP1;  同质粘附;  基因干扰;  食管癌    
Abstract:

Objective: To investigate the effect of IQGAP1 knockdown by RNA interference (RNAi) on homotypic cell-cell adhesion of esophageal carcinoma (EC) cells. Method: Western blot analysis was adopted to detect the protein expression of IQGAP1 in KYSE150 and EC9706 cells. Slow aggregation assay and cell dissociation assay were performed to detect the homotypic cell-cell adhesion properties of tumor cells. Further, KYSE150 and EC9706 cells were transfected with RNAi plasmid for targeting IQGAP1 gene or negative control, and then the cell lines of IQGAP1 knockdown were screened with G418. Level of IQGAP1 protein in interference group and control group was measured by Western blot, and the changes of homotypic cell-cell adhesion were observed by slow aggregation assay and cell dissociation assay. Result: Compared with EC9706 cells, level of IQGAP1 protein in KYSE150 cells was lower, while the homotypic cell-cell adhesion in KYSE150 cells was stronger. Further, Western blotting analysis showed that IQGAP1 protein expression was efficiently downregulated by stable transfection with IQGAP1 siRNA expression vector. The homotypic cell-cell adhesion was obviously enhanced by IQGAP1 knockdown. Conclusion: These data suggest that downregulation of IQGAP1 expression could significantly enhance homotypic cell-cell adhesion and decrease malignant phenotype of EC cells.
Key words: IQGAP1    Homotypic cell-cell adhesion    RNAi    Esophageal carcinoma
收稿日期: 2014-07-09 出版日期: 2014-09-25
ZTFLH:  R734.2  
基金资助:

国家自然科学基金（81372676）；中国博士后基金（2014M551058）资助项目
通讯作者: 王晓霞     E-mail: wxiaoxia99007@126.com
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引用本文:

王康, 贺春, 高伟, 王斌全, 王晓霞. IQGAP1基因干扰对人食管癌细胞同质粘附能力的影响[J]. 中国生物工程杂志, 2014, 34(9): 24-30.

WANG Kang, HE Chun, GAO Wei, WANG Bin-quan, WANG Xiao-xia. Effect of IQGAP1 Knockdown on Homotypic Cell-Cell Adhesion of Esophageal Carcinoma Cells. China Biotechnology, 2014, 34(9): 24-30.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140905    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I9/24


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