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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2010, Vol. 30 Issue (05): 43-48    
研究报告     
食蟹猴MHC I类A型新等位基因序列分析
黄上洺1,凌飞1,蒋琼橙2,卓敏1
华南理工大学生物科学与工程学院 广州 510006
Sequence Analysis of Macaca fascicularis's New MHC I-A Alleles
HUANG Shang-ming,LING Fei,JIANG Qiong-cheng,ZHUO Min
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China
 全文: PDF(563 KB)   HTML
摘要:

采用自行设计的MHC I类A型基因的引物从10只食蟹猴扩增得到11个3 000bp左右MHC等位基因。通过在Blast比对之后,证实有7个Mafa-A等位基因,4个Mafa-AG等位基因,新的等位基因序列已递交GenBank。由于每个个体可以检测出多个不同的Mafa-A等位基因,这说明食蟹猴Mafa-A基因经过多次复制,并且推测Mafa-AG是Mafa-A衍生物。同时,研究了等位基因在个体中的分布和系统进化树分析。

关键词: 食蟹猴主要组织相容性复合体系统进化树    
Abstract:

Elevent's MHC class I alleles around 3000bp have been identified from 10 Macaca fascicularis. BLAST analysis showed that the novel alleles include 7 Mafa-A alleles and 4 Mafa-AG alleles, which had been submitted to GenBank. Different Mafa-A alleles have been identified in each sample, it can be inferred that Mafa-A alleles have been duplicated and Mafa-AG alleles are derivative of Mafa-A alleles. Based on the new alleles, we analyze the alleles distribution among the samples and the phylogenetic tree.

Key words: Macaca fascicularis    MHC    Phylogenetic tree
收稿日期: 2010-01-25 出版日期: 2010-05-25
基金资助:

国家“973”重点基础研究规划项目子项目(2006CB701500)、广东省自然科学基金博士启动基金(07300826)资助项目

通讯作者: 卓敏     E-mail: zhuomin@scut.edu.cn
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引用本文:

黄上洺 凌飞 蒋琼橙 卓敏. 食蟹猴MHC I类A型新等位基因序列分析[J]. 中国生物工程杂志, 2010, 30(05): 43-48.

HUANG Shang-Ming, LING Fei, JIANG Qiong-Chen, ZHUO Min. Sequence Analysis of Macaca fascicularis's New MHC I-A Alleles. China Biotechnology, 2010, 30(05): 43-48.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I05/43

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