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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2016, Vol. 36 Issue (5): 46-52    DOI: 10.13523/j.cb.20160507
研究报告     
麻疯树G蛋白γ亚基JcAGG3基因的克隆及表达分析
张敏, 田银帅, 胡晓乐, 徐莺, 陈放
四川大学生命科学学院 成都 610064
Cloning and Expression Analysis of JcAGG3, G-protein Gamma Subunitsthree Gene from Jatrophacurcas L.
ZHANG Min, TIAN Yin-shuai, HU Xiao-le, XU Ying, CHEN Fang
College of Life Sciences, Sichuan University, Chengdu 610064, China
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摘要:

从麻疯树cDNA中克隆到一个与拟南芥AGG3同源的基因,命名为JcAGG3。该基因开放阅读框为834bp,编码277个氨基酸,软件预测等电点为8.61,分子质量为30.514kDa。亚细胞定位预测显示其定位于细胞质膜上。在该基因的顺势作用元件上发现了与胚乳发育、激素调节、光响应和逆境胁迫相关的启动元件。荧光定量PCR(qPCR)检测发现, JcAGG3在根、茎、叶和种子中均有表达,在发育中的种子中表达量最高,幼叶中的表达量显著高于老叶,在茎中只检测到极微量的表达;对麻疯树的幼苗进行黑暗处理后JcAGG3基因表达显著下调,脱落酸(ABA)和干旱胁迫处理下JcAGG3表达量显著增加。

关键词: 种子大小麻疯树表达分析基因克隆AGG3    
Abstract:

An Arabidopsis thaliana AGG3 homologous gene was cloned from Jatrophacurcas L., which was named JcAGG3. JcAGG3 had an open reading frame of 834bp, encoding a protein of 277 amino acids. Its theoretical isoelectricand molecular weight point were 30.514kDa and 8.61 respectively. The protein was predictedlylocalised in plasma membrane.Through Biologysoftwareprediction analysis, a lot of promoter-binding elements related to the development of endosperm, hormonal regulation, light response and abiotic stresses were found in the cis-acting elements of the gene. The expression of JcAGG3 in different tissues and different development stages were examined using real-time polymerase chain reaction(RT-PCR). The results showed that JcAGG3 was expressed in root, stem, leaf and seed, with the highest expression in developing seed but least in stem. Young lealves had a significantly higher expression level than older leaves. After 6h dark treatment, the level of JcAGG3 expression was significantly reduced. However, under abscisicacid(ABA) and drought treatment, the level of JcAGG3 expression were increased.

Key words: AGG3    Seed size    Gene cloning    Expression analysis    Jatrophacurcas L.
收稿日期: 2015-12-03 出版日期: 2016-01-04
ZTFLH:  Q789  
基金资助:

国家"十二五"重大科技支撑计划资助项目(2011BAD22B08)

通讯作者: 陈放     E-mail: chenfang_scu@126.com
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引用本文:

张敏, 田银帅, 胡晓乐, 徐莺, 陈放. 麻疯树G蛋白γ亚基JcAGG3基因的克隆及表达分析[J]. 中国生物工程杂志, 2016, 36(5): 46-52.

ZHANG Min, TIAN Yin-shuai, HU Xiao-le, XU Ying, CHEN Fang. Cloning and Expression Analysis of JcAGG3, G-protein Gamma Subunitsthree Gene from Jatrophacurcas L.. China Biotechnology, 2016, 36(5): 46-52.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160507        https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I5/46

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