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S-2-氯丙酸脱卤酶在毕赤酵母中的重组表达 |
秦迎春, 杨立荣, 徐刚, 吴坚平 |
浙江大学化学工程与生物工程学系 杭州 310027 |
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Cloning and Expression of S-2-chloropropionic Acid Dehalogenase in Pichia pastoris |
QIN Ying-chun, YANG Li-rong, XU Gang, WU Jian-ping |
Department of Chemical Engineering and Biological Engineering, Zhejiang University, Hangzhou 310027, China |
引用本文:
秦迎春, 杨立荣, 徐刚, 吴坚平. S-2-氯丙酸脱卤酶在毕赤酵母中的重组表达[J]. 中国生物工程杂志, 2013, 33(8): 25-31.
QIN Ying-chun, YANG Li-rong, XU Gang, WU Jian-ping. Cloning and Expression of S-2-chloropropionic Acid Dehalogenase in Pichia pastoris. China Biotechnology, 2013, 33(8): 25-31.
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https://manu60.magtech.com.cn/biotech/CN/
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[1] Littlechild J A, Rye C A. Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii. Extremophiles, 2009, 13: 179-190. [2] Nakamura T, Yamaguchi A. Roles of K151 and D180 in L-2-haloacid dehalogenase from Pseudomonas sp. YL: Analysis by molecular dynamics and abinitio fragment molecular orbital calculations. Journal of Computational Chemistry, 2009, 30: 2625-2634. [3] Markus A, Klages U. Oxidation and dehalogenation of 4-chlorophenylacetate by a two-component enzyme system from Pseudomonas sp. strain CBS3. Journal of Bacteriology, 1984, 160(2): 618-621. [4] Li Y F, Kurihara T. Mass spectrometric analysis of the reactions catalyzed by L-2-haloacid dehalogenase mutants and implications for the roles of the catalytic amino acid residues. Journal of Molecular Catalysis B-Enzymatic, 2003, 23: 337-345. [5] Smith J M, Harrison K. Purification and characterization of D-2-haloacid dehalogenase from Pseudomonas putida strain Aj1/23. Journal of General Microbiology, 1990, 136: 881-886. [6] Ridder I S, Rozeboom H J. Three-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate. Journal of Biological Chemistry, 1997, 272: 33015-33022. [7] McFadden J M, Frehywot G L, Townsend C A. A flexible route to (5R)-Thiolactomycin, a naturally occurring inhibitor of fatty acid synthesis. Org Lett, 2002, 4(22): 3859-3862. [8] 林春娇. C2/C3卤代酸脱卤酶的筛选及克隆表达. 杭州: 浙江大学, 2011. Lin C J. Cloning, screening and expression of C2/C3 Dehalogenation halogenated acid enzymes. Hangzhou: Zhejiang University, 2011. [9] Tsang J S, Sam L. Cloning and characterization of a cryptic haloacid dehalogenase from Burkholderia cepacia MBA4. Journal of Bacteriology, 1999, 181(19): 6003. [10] Jiang K Y, Zhang B, Yan Y J. Cloning and expression of Pseudomonas fluorescens 26-2 lipase gene in Pichia pastoris and characterizing for transesterification. Applied Biochemistry and Biotechnology, 2009, 159: 355-365. [11] 彭毅, 步威, 康良仪. 甲醇酵母表达系统. 生物技术通报, 2000, 1: 38-41. Peng Y, Bu W, Kang L Y. Methanol yeast expression system. Biotechnology Bulletin, 2000, 1: 38-41. [12] Montesio R, Garcia R, Quintero O, et al. Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris. Protein Expression and Purification, 1998, 14(2): 197-207. [13] Gemmill T R, Trimble R B. Overview of N-and O-linked oligosaccharide structures found in various yeast sprices. Biochimica et Biophysica Acta, 1999, 1426(2): 227-237. [14] James M, Cregg, Kevin J,et al. Pichia pastoris as a host system for transformations. Molecular and Cellular Biology, 1985, 5(12): 3376-3385. [15] 项炯华, 吴坚平, 王能强, 等. 2-氯丙酸脱卤酶产酶菌种的筛选及酶学性质研究. 化学反应工程与工艺, 2005, 21(6): 537-541. Xiang J H, Wu J P, Wang N Q, et al. Strains screening and enzymology properties of 2-chloropropionic acid dehalogenase. Chemical Reaction Engineering and Technology, 2005, 21(6): 537-541. [16] 刘鹏, 林春娇, 杨立荣, 等. S-2-氯丙酸脱卤酶的定向进化及其应用. 中国生物工程杂志, 2012, 32(5): 66-72. Liu P, Lin C J, Yang L R, et al. Directed evolution and application of S-2-chloropropionic acid dehalogenase. China Biotechnology, 2012, 32(5): 66-72. [17] 黄培堂. 分子克隆实验指南. 第3版. 北京: 科学出版社, 2005. Huang P T. Molecular Cloning (A laboratory Manual). 3 ed, Beijing: Science Press, 2005. [18] Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680-685. [19] Chen X L, Cao Y H, Ding Y H, et al. Cloning, functional expression and characterization of Aspergillus sulphureus β-mannanase in Pichia pastoris. Biotechnol, 2007, 128: 452-461. [20] Gorgenes J F, Van W H, Knoetze J H, et al. Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium. Applied Microbiology and Biotechnology, 2005, 67: 684-691. [21] KatakuraY, Zhang W, Zhuang G, et al. Effect of methanol concentration on the production of human β2-glycoprotein I domain V by a recombinant Pichia pastoris: A simple system for the control of methanol concentration using a semiconductor gas sensor. Journal of Fermentation and Bioengineering, 1998, 86: 482-487. |
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