| 农业生物技术专栏 |
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| 转基因玉米中植酸酶蛋白免疫亲和纯化体系的建立 |
| 赵倩倩1, 周晓今1, 柳小庆1, 杨文竹1, 李素贞2, 刘奇3, 陈茹梅1 |
1. 中国农业科学院生物技术研究所 作物功能基因组与遗传改良中心 北京 100081;
2. 河北农业大学 保定 071001;
3. 北京奥创金标生物技术有限公司 北京 102600 |
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| Purification of Phytase from Transgenic Maize by Immunoaffinity Chromatography |
| ZHAO Qian-qian1, ZHOU Xiao-jin1, LIU Xiao-qing1, YANG Wen-zhu1, LI Su-zhen2, LIU Qi3, CHEN Ru-mei1 |
1. Crop functional genome and genetic improvement department, Biotechnology institute, CAAS, Beijing 100081, China;
2. Agricultural University of Hebei, Baoding 071001, China;
3. Beijing Artron Biotech Co., Ltd., Beijing 102600, China |
引用本文:
赵倩倩, 周晓今, 柳小庆, 杨文竹, 李素贞, 刘奇, 陈茹梅. 转基因玉米中植酸酶蛋白免疫亲和纯化体系的建立[J]. 中国生物工程杂志, 2013, 33(6): 125-130.
ZHAO Qian-qian, ZHOU Xiao-jin, LIU Xiao-qing, YANG Wen-zhu, LI Su-zhen, LIU Qi, CHEN Ru-mei. Purification of Phytase from Transgenic Maize by Immunoaffinity Chromatography. China Biotechnology, 2013, 33(6): 125-130.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
或
https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I6/125
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[9] 闫广为, 陈茹梅, 石鹏君,等. 转基因玉米中植酸酶蛋白沉淀条件探索. 中国农业科技导报, 2009, 11(4): 118-122. Yan G W, Chen R M, Shi P J, et al. Studies on precipitation conditions of phytase protein from transgenic corn. Journal of Agricultural Science and Technology, 2009,11(4): 118-122.
[10] 姚斌,袁铁铮,范云六,等. 来源于Bacillus subtilis的中性植酸酶基因的克隆及在大肠杆菌中的表达. 生物工程学报, 2001,170(1): 11-15. Yao B, Yuan T Z, Fang Y L,et.al. Cloning of neutral phytase gene nphy from Bacillus subtilis and its expression in Escherichia coli. Chin J Biotechnol, 2001,17(1):11-15.
[11] Spier M R, Fendrich R C, Almeida P C, et al. Phytase produced on citric byproducts: purification and characterization. World Journal of Microbiology and Biotechnology, 2010, 27: 267-274.
[12] Dionisio G, Madsen C K, Holm P B, et al. Cloning and characterization of purple acid phosphatase phytases from wheat, barley, maize, and rice. Physiol, 2011, 156: 1087-1100. |
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