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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2012, Vol. 32 Issue (6): 7-12    
研究报告     
小鼠胚胎干细胞对黑色素瘤细胞体外生物学行为的影响
张小梅, 方廖琼, 王智彪
重庆医科大学生物医学工程学院 省部共建超声医学工程国家重点实验室 超声医学工程重庆市市级重点实验室 重庆 400016
Influence of Mouse Embryonic Stem Cells on Melanoma Cells in vitro
ZHANG Xiao-mei, FANG Liao-qiong, WANG Zhi-biao
State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology Chongqing Key Laboratory of Ultrasound in Medicine and Engineering Department of Biomedical Engineering Chongqing Medical University, Chongqing 400016, China
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摘要: 探讨体外共培养环境中小鼠胚胎干细胞对小鼠黑色素瘤B16细胞的影响。建立C57BL/6小鼠胚胎干细胞系,通过小鼠胚胎干细胞与肿瘤细胞体外共培养模型观察小鼠胚胎干细胞对肿瘤细胞的形态及生长行为的影响,MTT法与transwell小室法分别检测共培养后肿瘤细胞粘附性、迁移性及侵袭性的变化。共培养中小鼠胚胎干细胞能够侵入并推开小鼠黑色素瘤细胞形成自己的生长空间,与对照组比较共培养后肿瘤细胞的粘附性、迁移性及侵袭性均显著降低(P<0.05,P<0.01)。结果表明体外共培养体系中小鼠胚胎干细胞能够侵袭肿瘤细胞,并降低细胞粘附、迁移及侵袭相关恶性生物学行为。
关键词: 共培养黏附侵袭迁移    
Abstract: To investigate the impacts of mouse embryonic stem cells on mouse melanoma B16 cells in vitro co-cultured environment. C57BL / 6 mouse embryonic stem cell lines were established.The effects of mouse embryonic stem cells on tumor cell morphology and growth behavior were observed with establishing a co-culture system in vitro.MTT assay and transwell chamber assay were used to detect change of the co-cultured tumor cell adhesion, migration and invasiveness. Mouse embryonic stem cells could invade and push the mouse melanoma cells to form their own room for growth in co-culturing. The adhesion, migration and invasion ability of the tumor cells after co-cultured with mouse embryonic stem cells significantly declined(P<0.05,P<0.01). The results implied that the co-culture mouse embryonic stem cells could invade tumor cells and inhibit their related malignant behaviors like adhesion,migration and invasion ability.
Key words: Co-culture    Adhesion    Migration    Invasion
收稿日期: 2012-03-26 出版日期: 2012-06-25
ZTFLH:  Q2-33  
基金资助: 国家"973"计划(2011CB707902)资助项目
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引用本文:

张小梅, 方廖琼, 王智彪. 小鼠胚胎干细胞对黑色素瘤细胞体外生物学行为的影响[J]. 中国生物工程杂志, 2012, 32(6): 7-12.

ZHANG Xiao-mei, FANG Liao-qiong, WANG Zhi-biao. Influence of Mouse Embryonic Stem Cells on Melanoma Cells in vitro. China Biotechnology, 2012, 32(6): 7-12.

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https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I6/7

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