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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2016, Vol. 36 Issue (7): 27-33    DOI: 10.13523/j.cb.20160705
研究报告     
土生隐球酵母14-3-3蛋白耐铝能力的研究
刘帅, 邱金奎, 年洪娟
昆明理工大学生命科学与技术学院 昆明 650500
Study on Aluminum Resistant Capacity of 14-3-3 Protein from Cryptococcus humicola
LIU Shuai, QIU Jin-kui, NIAN Hong-juan
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
 全文: PDF(994 KB)   HTML
摘要:

14-3-3蛋白家族是由多个高度保守的成员构成的调节性蛋白质家族,它们主要以磷酸化的形式与伴侣蛋白相互作用,并能够以多种方式来影响靶蛋白。通过构建14-3-3蛋白原核表达载体,纯化重组蛋白获得14-3-3蛋白抗体。为了验证14-3-3蛋白基因在耐铝中的作用,构建14-3-3酵母表达载体,得到14-3-3过表达酵母菌株。在5mmol/L铝浓度下,转基因酵母比对照酵母长势好,这表明14-3-3蛋白通过促进生长赋予酵母对铝胁迫的耐受性。

关键词: 耐铝土生隐球酵母14-3-3蛋白    
Abstract:

14-3-3 proteins are a family of conserved proteins that interact with numerous partner proteins in a phospho-specific manner, and can affect the target proteins in a variety of ways. prokaryotic expression vector of 14-3-3 gene was constructed, and the recombinant 14-3-3 protein was purified, then the 14-3-3 antibody was obtained using this recombinant protein. To characterize the aluminum (Al) tolerance of 14-3-3 protein in yeast, a transgenic Saccharomyces cerevisiae containing pYES3/CT-14-3-3 was generated. The results of growth showed that the transgenic yeast grew better than the control strain on plates with 5mmol/L Al. These results suggest that 14-3-3 conferred Al tolerance in yeast by promoting the growth of yeast.

Key words: Aluminum resistance    Cryptococcus humicola    14-3-3 protein
收稿日期: 2016-01-12 出版日期: 2016-03-16
ZTFLH:  Q816  
基金资助:

国家自然科学基金资助项目(31560246,31160020)

通讯作者: 年洪娟     E-mail: hjnian@163.com
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引用本文:

刘帅, 邱金奎, 年洪娟. 土生隐球酵母14-3-3蛋白耐铝能力的研究[J]. 中国生物工程杂志, 2016, 36(7): 27-33.

LIU Shuai, QIU Jin-kui, NIAN Hong-juan. Study on Aluminum Resistant Capacity of 14-3-3 Protein from Cryptococcus humicola. China Biotechnology, 2016, 36(7): 27-33.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160705        https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I7/27

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