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构建尿嘧啶磷酸核糖转移酶基因缺失菌株实现Gluconobacter suboxydans基因组无痕修饰 |
杜红燕, 李天明, 刘金雷, 冯惠勇 |
河北科技大学生物科学与工程学院 石家庄 050018 |
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Construct the Uracil Phosphoribosyl Transferase Gene Mutant Strain in Gluconobacter suboxydans for Seamless Genome Editing |
DU Hong-yan, LI Tian-ming, LIU Jin-lei, FENG Hui-yong |
College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China |
引用本文:
杜红燕, 李天明, 刘金雷, 冯惠勇. 构建尿嘧啶磷酸核糖转移酶基因缺失菌株实现Gluconobacter suboxydans基因组无痕修饰[J]. 中国生物工程杂志, 2016, 36(7): 64-71.
DU Hong-yan, LI Tian-ming, LIU Jin-lei, FENG Hui-yong. Construct the Uracil Phosphoribosyl Transferase Gene Mutant Strain in Gluconobacter suboxydans for Seamless Genome Editing. China Biotechnology, 2016, 36(7): 64-71.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160710
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https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I7/64
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[1] De Ley J,Swings J,Gossele F.The Genus Gluconobacter.In:Krieg N R,Holt J G.Bergey's Manual of Systematic Bacteriology,Baltimore:Williams&Wilkins Co,1984:267-278.
[2] Deppenmeier U,Hoffmeister M,Prust C.Biochemistry and biotechnological applications of Gluconobacter strains.Applied and Molecular Biotechnology.2002,60(3):233-242.
[3] Reichstein T,Grü ssner A.Eine ergiebige synthese der 1-ascorbinsäure (C-vitamin).Helvetica Chimica Acta 1934,17(1):311-328.
[4] Michael S.Regioselective oxidation of aminosorbitol with Gluconobacter oxydans,key reaction in the industrial synthesis of 1-deoxynojirimycin syntheis.Journal of Psychiatry&Neuroscience Jpn,2004,29(5):364-382.
[5] Adachi O,Moonmangmee D,Toyama H,et al.New developments in oxidative fermentation.Applied and Molecular Biotechnology,2003,60(6):643-653.
[6] Liu P,Jenkins N A,Copeland N G.A highly efficient recombineering-based method for generating conditional knockout mutations.Genome Research,2003,13(3):476-484.
[7] 杨奇.大肠杆菌DH5α upp基因的敲除及其应用研究.南京:南京理工大学,环境与生物工程学院,2013.Yang Q.Knockout of the upp gene in Escherichia coli DH5α and its application research.Nanjing:Nanjing University of Science&Technology,Environmental and Biological Engineering,2013.
[8] Bailey J E.Toward a science of metabolic engineering.Science,1991,252(5013):1668-1675.
[9] 胡逢雪,丁锐,崔震海,等.大肠杆菌基因无痕敲除技术及策略.生物技术通讯,2013,24(4):552-557.Hu F X,Ding R,Cui Z H,et al.Approaches and strategies of gene scarless knockout in the Escherichia coli genome.Letters In Biotechnology,2013,24(4):552-557.
[10] Hasegawa N,Abeil M K,Yokoyama K,et al.Cyclophosphamide enhances antitumor efficacy of oncolytic adenovirus expressing uracil phosphoribosyltransferase (UPRT) in immunocompetent Syrian hamsters.International Journal of Cancer,2013,133(6):1479-1489.
[11] Andersen P S,Smith J M,Mygind B.Characterization of the upp gene encoding uracil phosphoribosyltransferase of Escherichia coli K12.Federation of European Biochemical Societies,1992,204(1):51-56.
[12] Tan Z G,Zhu X N,Chen J,et al.Activating phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in combination for improvement of succinate production.Applied and Environmental Microbiology,2013,79(16):4838-4844.
[13] Jantama K,Zhang X L,Moore J C,et al.Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C.Biotechnology and Bioengineering,2008,101(5):881-893.
[14] Zhang X L,Jantama K,Moore J C,et al.Production of L-alanine by metabolically engineered Escherichia coli.Applied Genetics And Molecular Biotechnology,2007,77(2):355-366.
[15] Michael E K,Philip H E,Steve D H,et al.Four new derivatives of the broad-host-range cloning vector pBBR1MCS,carring different antibiotic-resistance cassettes.Gene,1995,166(1):175-176.
[16] Prust C,Hoffmeister M,Liesegang H,et al.Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans.Nature Biotechnology,2005,23(2):195-200.
[17] 余华,熊浚智,何晓梅,等.采用Red重组系统敲除铜绿假单胞菌弹性蛋白酶基因.中国人兽共患病学报,2013,2:129-132,137.Yu H,Xiong J Z,He X M,et al.Generation of a Pseudomonas aeruginosa elastase gene targeted deletion mutant by Red recombination system.Chinese Journal of Zoonoses,2013,2:129-132,137.
[18] Lim J H,Seo S W,Kim S Y,et al.Refactoring redox cofactor regeneration for high-yield biocatalysis of glucose to butyric acid in Escherichia coli.Bioresource Technology,2013,135:568-573.
[19] Koresawa Y K,Miyagawa S J,Ikawa M H,et al.Synthesis of a new cre recombinase gene based on optimal codon usage for mammalian system.The Journal of Biochemistry,2000,127(3):367-372.
[20] Zhu D L,Zhao K,Xu H J,et al.Construction of thyA deficient Lactococcus lactis using the Cre-loxP recombination system.Annals of Microbiology,2015,66(3):1659-1665.
[21] Jager W,Schafer A,Puhler A,et al.Expression of the Bacillus subtilis SacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans.Journal of Bacteriology,1992,174(16):5462-5465.
[22] Hu F,Jiang X,Zhang J J,et al.Construction of an engineered strain capable of degrading two isomeric nitrophenols via a sacB-and gfp-based markerless integration system.Applied and Molecular Biotechnology,2014,98(10):4749-4756.
[23] Tan Y Z,Xu D Q,Li Y,et al.Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum.Plasmid,2012,67(1):44-52. |
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