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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (5): 66-74    DOI: 10.13523/j.cb.20140509
技术与方法     
一株病原拮抗野生菌株的筛选、鉴定及其发酵工艺优化
韩启灿, 霍光华, 罗桂祥
江西农业大学生物科学与工程学院 南昌 330045
Screening, Identification and Fermentation Process Optimization of a Wild Fungus Against Pathogens
HAN Qi-can, HUO Guang-hua, LUO Gui-xiang
College of Bioscience And Bioengineering of Jiangxi Agricultural University, Nancang 330045, China
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摘要：

为获得广谱抗菌功能野生菌株并提高其发酵产物中抗菌物质的含量。采用管碟法和菌丝生长速率法筛选功能菌株，ITS序列分析鉴定功能菌株，通过响应面法和正交设计优化发酵生产抗菌物质的工艺。筛选到一株强效、广谱抗菌功能菌株，鉴定为Cerrena sp.，其发酵产物对金黄色葡萄球菌、大肠杆菌、白色念珠菌、枯草芽孢杆菌和水稻纹枯病菌有显著拮抗作用。该菌株的摇甁发酵配方及培养条件为：马铃薯13.99 g/L，蔗糖 41.58 g/L，VB1 0.027 g/L，麸皮7 g/L，KH2PO4 2 g/L，MgSO4·7H2O 2 g/L；摇床温度28 ℃、发酵周期10 d、种龄4 d、接种量8%、初始pH为5.0、装液量110 ml/250 ml。该菌株有明显抑菌活性，发酵工艺优化后抗菌活性提高了30.37%，为该菌株今后的应用、抗菌剂的分离提纯和产业化提供了实验依据。
关键词： 野生大型真菌;  病原菌;  发酵工艺;  响应面法    
Abstract:

To obtain wild mushrooms having broad-spectrum antimicrobial activity and enhance the antimicrobial compounds productivity were our purpose. The active strains were screened in terms of the methods of well diffusion assay and hypha growth rate. the fermentation parameters for antimicrobial compounds productivity were optimized by response surface analysis and orthogonal test. One white-rot fungus 0331 with broad-spectrum antagonistic to pathogens was obtained, and it was identified as Cerrena sp. by ITS-5.8 rDNA sequences analysis. The fermentation broth showed the antimicrobial activity against Staphylococcus aureus, Escherichia coli, Monilia albican, Bacillus subtilis and Rhizoctnia solani. The optimal medium for 0331 was composed of (in g/L): potatoes 13.99, wheat bran 7.00, Sucrose 41.58, KH2PO4 2.00, MgSO4·7H2O 2.00, VB1 0.027. The optimal culture conditions were as follows: the initial pH value of medium 5.0, the fermentation period 10 d, packing volume 110ml/250ml, inoculation 8mL/100ml, rotate speed 180r/min, temperature 28℃, respectively. The 0331 strain showed obvious antimicrobial activity, with an increase of 30.37% compared to the original fermentation parameters.
Key words: Wild macrofungi    Pathogens    Fermentation process    Response surface methodology
收稿日期: 2014-03-05 出版日期: 2014-05-25
ZTFLH:  Q815  
通讯作者: 霍光华     E-mail: hgh3813899@sohu.com
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引用本文:

韩启灿, 霍光华, 罗桂祥. 一株病原拮抗野生菌株的筛选、鉴定及其发酵工艺优化[J]. 中国生物工程杂志, 2014, 34(5): 66-74.

HAN Qi-can, HUO Guang-hua, LUO Gui-xiang. Screening, Identification and Fermentation Process Optimization of a Wild Fungus Against Pathogens. China Biotechnology, 2014, 34(5): 66-74.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140509    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I5/66


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