技术与方法 |
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耐底物腈水合酶融合子的产酶条件优化 |
张丽铃1,2, 王筱兰1,2 |
1. 江西师范大学生命科学学院 南昌 330022; 2. 功能有机小分子教育部重点实验室 南昌 330022 |
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Optimization Fermentation Conditions of the Nitrile Hydratase Fusant Strain Tolerance to the Substrate |
ZHANG Li-ling1,2, WANG Xiao-lan 1,2 |
1. School of life Science ,Jiangxi Normal University, Nanchang 330022,China; 2. Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Nanchang 330022,China |
[1] Nowak W, Ohtsuka Y, Hasegawa J, et al. Density functional study on geometry and electronicstructure of nitrilehy drataseactive sitemodel. International Journal of Quantum Chemistry, 2002,90(3):1174-1187. [2] 褚以文.微生物培养基优化方法及其OPTI 优化软件.国外医药抗生素分册,1999,20 (2):58-61. Chu Y W.The optimization of microbial culture medium and OPTI optimization software. World Notes on Antibiotics,1999,20(2):58-61. [3] 张超,张克峰,侯书国,等.利用响应面法优化ε-聚赖氨酸发酵培养基.微生物学通报,2011,38(5) :641-646. Zhang C,Zhang K F, Hou S G. Medium optimization of ε-polylysine production by response surface analysis. Microbiology China, 2011,38(5) :641-646. [4] 国家技术监督局.聚丙烯酰胺中残留丙烯酰胺含量测定方法———溴化法.GB12005.3-89.北京:中国标准出版社,1990.9-11. State Bureau of Technical Supervision.Determination for Residual Acrylamide of Polyacrylamide—Bromating Method. GB12005.3-89. Beijing: Standards Press of China,1990.9-11. [5] Montgomery D C. Design and Analysis of Experiments.New York: John Wiley & Sons, 1991.45-50. [6] Qian F P, Wang H G. Study of the filtration performance of a plain wavefabric filter using response surface methodology. J Hazard Mater, 2010,176: 559-568. |
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