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芽孢杆菌β-折叠桶植酸酶的原核可溶性表达优化及包涵体复性研究 |
李倩倩1, 李中媛2, 冯舵1, 黄火清2, 韩翠晓1, 杨培龙2, 姚斌2, 高伟1 |
1. 北京林业大学理学院/林木育种国家工程实验室 北京 100083; 2. 中国农业科学院饲料研究所农业部饲料生物技术重点实验室 北京 100081 |
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Optimizing Soluble Expression and Inclusion Body Renature Research of β-Propeller Phytase of Bacillus sp. HJB17 in E.coli |
LI Qian-qian1, LI Zhong-yuan2, FENG Duo1, HUANG Huo-qing2, HAN Cui-xiao1, YANG Pei-long2, YAO Bin2, GAO Wei 1 |
1. National Engineering Laboratory for Tree Breeding /College of Science,Beijing Forestry University,Beijing 100083,China; 2. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences, Beijing 100081,China |
引用本文:
李倩倩, 李中媛, 冯舵, 黄火清, 韩翠晓, 杨培龙, 姚斌, 高伟. 芽孢杆菌β-折叠桶植酸酶的原核可溶性表达优化及包涵体复性研究[J]. 中国生物工程杂志, 2012, 32(08): 49-55.
LI Qian-qian, LI Zhong-yuan, FENG Duo, HUANG Huo-qing, HAN Cui-xiao, YANG Pei-long, YAO Bin, GAO Wei. Optimizing Soluble Expression and Inclusion Body Renature Research of β-Propeller Phytase of Bacillus sp. HJB17 in E.coli. China Biotechnology, 2012, 32(08): 49-55.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
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https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I08/49
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[1] Mullaney E J, Daly C B, Ullah A H. Advances in phytase research.Adv Appl Microbiol,2000(47):157-199. [2] Pandey A,Szakacs G,Soccol C R,et al.Production,purification and properties of microbial phytases.Bioresource Technology,2001,77(3):203-214. [3] Haefner S,Knietsch A,Scholten E,et al.Biotechnological production and applications of phytases.Appl Microbiol Biotechnol,2005,68(5):588-597. [4] Bohn L,Meyer A S,Rasmussen S K.Phytate:impact on environment and human nutrition.A challenge for molecular breeding.J Zhejiang Univ Sci B,2008 9(3):165-191. [5] Lei X,Porres J,Mullaney E,et al.Phytase:source,structure and application.In:Polaina J, MacCabo A P, Eds. Industrial Enzymes Structure, Function and Applications. Netherlands: Springer, 2007.505-529. [6] Baruah K, Pal A K, Sahu N P,et al.Dietary protein level,microbial phytase,citric acid and their interactions on bone mineralization of Labeo-rohita (Hamilton) juveniles.Aquacult Res, 2005,36(8),803-812. [7] Lei X G, Stahl C H.Biotechnological development of effective phytases for mineral nutrition and environmental protection.Appl Microbiol Biotechnol,2001,57(4):474-481. [8] Ha N C,Oh B C,Shin S,et al.Crystal structures of a novel,thermostable phytase in partially and fully calcium-loaded states.Nature Struct Biol,2000,7:147-153. [9] Fu S,Sun J,Qian L.Effect of Ca2+ on beta-propeller phytases.Protein Pept Lett,2008,15(1):39-42. [10] Huang H Q,Shao N,Wang Y R,et al.A novel beta-propeller phytase from Pedobacter nyackensi MJ11 CGMCC 2503 with potential as an aquatic feed additive.Appl Microbiol Biotechnol,2009,83(2):249-259. [11] Bradford M M.A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem,1976,72: 248-254. [12] Choi Y M,Suh H J,Kim J M.Purification and properties of extracellular phytase from Bacillus sp. KHU-10. J Protein Chem,2001,20(4):287-292. [13] Rao D E,Rao K V,Reddy V D.Cloning and expression of Bacillus phytase gene (phy)in Escherichia coli and recovery of active enzyme from the inclusion bodies.J Appl Microbiol,2008,105(4):1128-1137. [14] Hartl F U,Hayer-Hartl M.Molecular chaperones in the cytosol from nascent chain to folded protein.Science,2002,295(5561):1852-1858. |
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