研究报告 |
|
|
|
|
金发草LEA 3 基因两个剪接体转化酿酒酵母的抗非生物胁迫功能分析 |
李锐1, 王文国2, 范林洪1, 王胜华1 |
1. 四川大学生命科学学院 生物资源与生态环境教育部重点实验室 成都 610064; 2. 农业部沼气科学研究所 生物质能技术研究中心 成都 610041 |
|
Abiotic Stress Tolerance Analysis Two Alternatively Spliced Isoforms of LEA3 Gene from Pogonatherum paniceum in Yeast |
LI Rui1, WANG Wen-guo2, FAN Lin-hong1, WANG Sheng-hua 1 |
1. College of Life Sciences, Sichuan University, Department of Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu 610064, China; 2. Research Centre of Biomass Energy Technology, Biogas Institute of Ministry of Agriculture,Chengdu 610041, China |
引用本文:
李锐, 王文国, 范林洪, 王胜华. 金发草LEA 3 基因两个剪接体转化酿酒酵母的抗非生物胁迫功能分析[J]. 中国生物工程杂志, 2012, 32(08): 30-35.
LI Rui, WANG Wen-guo, FAN Lin-hong, WANG Sheng-hua. Abiotic Stress Tolerance Analysis Two Alternatively Spliced Isoforms of LEA3 Gene from Pogonatherum paniceum in Yeast. China Biotechnology, 2012, 32(08): 30-35.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
或
https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I08/30
|
[1] Ast G. How did alternative splicing evolve? Nature Reviews Genetics, 2004,5:773-782. [2] Tazi J, Bakkour N Stamm. Alternative splicing and disease. Biochimica et Biophysica Acta-Molecular Basis of Disease, 2009,1792 (1):14-26. [3] Dircksen H. Insect ion transport peptides are derived from alternatively spliced genes and differentially expressed in the central and peripheral nervous system. Journal of Experimental Biology, 2009,212(3):401-412. [4] 曾纪晴, 张明永. 可变剪接在植物逆境相关基因表达调控中的作用. 植物生理学通讯, 2006,42(6):1005-1014. Zeng J Q, Zhang M Y. The role of alternative splicing in the regulation of plant stress-associated gene expression. Plant Physiology Communications, 2006,42(6):1005-1014. [5] Dure L. A repeating 11-mer amino acid motif and plant desiccation. Plant J, 1993,3:363-369. [6] Garay-Arroyo A, Colmenero-Flores J M, Garciarrubio A, et al. Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem, 2000,275:5668-5674. [7] Wise M J, Tunnacliffe A. POPP the question: what do LEA proteins do? Trends Plant Sci, 2004,9:13-17. [8] Wise M J, LEAping to conclusions: a computational reanalysis of late embryogenesis abundant proteins and their possible roles. BMC Bioinform, 2003,4:52. [9] Liu Y, Zheng Y. PM2, a group 3 LEA protein from soybean, and its 22-mer repeating region confer salt tolerance in Escherichia coli. Biochem Biophys Res Commun, 2005,331:325-332. [10] Tunnacliffe A, Wise M J. The continuing conundrum of the LEA proteins. Naturwissenschaften, 2007,94:791-812. [11] Gellissen G, Melber K, Janowicz Z A, et al. Heterologous protein production in yeast.Anton Van Leeuwenhock, 1992,62:79-93. [12] 王文国. 金发草LEA3基因与水稻Rad9基因的选择性剪接与基因功能研究. 成都:四川大学,生命科学学院,2010. Wang W G. Alternative splicing and gene functional analysis of LEA3 gene in Pogonatherum paniceum and Rad9 gene in rice. Chengdu:Sichuan University, College of Life Science, 2010. [13] 邓成菊, 张建斌, 贾彩红, 等. 香蕉乙二醛酶基因增强酿酒酵母对非生物胁迫抵抗能力的研究. 中国生物工程杂志, 2010, 30(8):22-26. Deng C J, Zhang J B, Jia C H, et al. Enhancement of tolerance to abiotic stress of Saccharomyces cerevisiae transformed by a gene encoding glyoxalase from banana. China Biotechnology, 2010,30(8):22-26. [14] Ginger A S, William R, Marcotte J. The wheat LEA protein Em function as an osmoprotective molecule Sacharomyces cerevisiae. Plant Mol Biol, 1999, 39(1):117-128. [15] Gal Z T, Glazer I, Koltai H. An LEA group 3 family member is survival of C. elegans during exposure to stress. FEBS Lett, 2004, 577:21-26. [16] 汤晓倩, 于丽霞, 武晓璐, 等. 第三组胚胎晚期丰富蛋白lea3基因研究概述. 生命科学, 2010,22(6):551-555. Tang X Q, Yu L X, Wu X L, et al. Research advance in the group three of late-embryogenesis-abundant proteins and genes. Chinese Bulletin of Life Sciences, 2010,22(6):551-555. [17] Wang B F, Wang Y C, Zhang D W, et al. Verification of the resistance of a LEA gene from Tamarix expression in Saccharomyces cerevisiae to abiotic stresses. Journal of Forestry Research, 2008,19(1):58-62. [18] 郭小勤, 李德葆.植物前体mRNA的选择性剪接.农业生物技术学报,2006,14(5):809-815. Guo X Q, Li D B. Pre-mRNA alternative splicing in plants. Journal of Agriculture Biotechnology,2006,14(5):809-815. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|