响应面法优化产XRN1蛋白发酵培养基

中国生物工程杂志

2013, Vol. 33

Issue (4): 121-128

技术与方法

响应面法优化产XRN1蛋白发酵培养基

吴迎春, 马琴琴, 丁先锋, 张凯, 刘立丽, 郭江峰

浙江理工大学生命科学学院杭州 310018

Expression of XRN1 Protein and Optimization of Fermentation Medium with Response Surface Method

WU Ying-chun, MA Qin-qin, DING Xian-feng, ZHANG Kai, LIU Li-li, GUO Jiang-feng

College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

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摘要： 应用Plackett-Burman(PB)设计法对影响XRN1蛋白表达的发酵培养基组分进行优化,选取的8个相关因子为酵母提取物、硫酸钙、MgSO₄·7H₂O、甘油、硫酸钾、磷酸、硫酸铵和氢氧化钾。统计分析表明,影响XRN1蛋白表达的关键因子为酵母提取物、硫酸镁和硫酸钾。在进行最陡爬坡试验逼近3个关键因素的最大响应区域的基础上,采用Box-Behnken Design法对发酵培养基组分进行优化,通过响应面法分析最佳条件为酵母提取物0.45%、硫酸镁0.38%和硫酸钾1.4%。在最佳培养条件下,工程菌生物量增加约0.5倍,蛋白表达量提高近40%。

关键词： XRN1蛋白; 基础盐培养基; 响应面分析

Abstract: Response surface method (RSM) was used to optimize the fermentation medium of XRN1 protein expression. The Plackett-Burman design was used to evaluate the effects of eight factors, including yeast extract, calcium sulfate, MgSO₄?7H₂O, glycerol, potassium sulfate, phosphoric acid, ammonium sulfate and potassium hydroxide. The statistical analysis revealed that the key factors influencing XRN1 protein expression were yeast extract, magnesium sulfate and potassium sulfate. The path of steepest ascent was used to define optimal response region for these three factors, then the optimal level combinations of factors were obtained through Box-Behnken design, the optimal conditions were as follows: yeast extract 0.45%, magnesium sulfate 0.38%, and potassium sulfate 1.4%. Under optimal conditions, biomass of yeast could increase by 0.5 fold and expression of XRN1 protein could increase by 40%.

Key words: XRN1 protein Base salt medium Response surface method

收稿日期: 2012-10-12 出版日期: 2013-04-25

ZTFLH:

Q819

基金资助: 国家自然科学基金(11105121);浙江省重点科技创新团队项目(2012R10033-07);浙江省教育厅高校科研计划(Y201226241)资助项目

通讯作者: 郭江峰 E-mail: jfguo@zstu.edu.cn

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引用本文:

吴迎春, 马琴琴, 丁先锋, 张凯, 刘立丽, 郭江峰. 响应面法优化产XRN1蛋白发酵培养基[J]. 中国生物工程杂志, 2013, 33(4): 121-128.

WU Ying-chun, MA Qin-qin, DING Xian-feng, ZHANG Kai, LIU Li-li, GUO Jiang-feng. Expression of XRN1 Protein and Optimization of Fermentation Medium with Response Surface Method. China Biotechnology, 2013, 33(4): 121-128.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I4/121

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