赤霉素发酵与大孔树脂吸附过程耦合工艺优化

中国生物工程杂志

2012, Vol. 32

Issue (11): 70-74

研究报告

赤霉素发酵与大孔树脂吸附过程耦合工艺优化

王卫, 李忠海, 黎继烈, 曾泊全

中南林业科技大学生命科学与技术学院长沙 410004

Optimization of Fermentation Process for Gibberellic Acid Production Coupling with Adsorptive Extraction by Macroporous Resin

WANG Wei, LI Zhong-hai, LI Ji-li, ZENG Bo-quan

College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China

全文: PDF(634 KB) HTML

摘要： 赤霉素生物合成过程中存在产物抑制,采用发酵与提取耦合技术将减弱产物抑制,提高赤霉素生产效率。对多种国产大孔树脂进行赤霉素GA₃静态吸附、解析实验,筛选出性能较优的大孔树脂D-100;并采用单因素和响应面分析方法优化D-100树脂添加至赤霉素发酵的工艺条件。实验数据表明:大孔树脂D-100的最适添加时间与最适树脂量分别为71h和2.2%,发酵所得赤霉素总量为221.75mg,赤霉菌平均比生产速率为0.96mg/(g·h)。与常规发酵比较,耦合发酵所得赤霉素总量提高了1.496倍。

关键词： 赤霉素; 大孔树脂; 吸附; 耦合

Abstract: The mechanism of end product inhibition exists in gibberellin acid (GA₃) biosynthesis of mycelium of Gibberella fujikuroi. Fermentation technology coupling with resin extraction would decrease the product inhibition and enhance the efficiency of GA₃ production. Four macroporous resins were tested for their static adsorption/desorption performance towards GA₃, and D-100 resin was selected as adsorbent through the method compared the adsorption capacity of the GA₃ and elution efficiency. The facts about addition time and the amount of resin were studied by regression and response surface analysis. The experimental results showed that the optimal addition time and amount of resin were 70.75h and 2.02%, accordingly. The total gibberellin productivity and average specific production rate in the fermentation was 221.75mg and 0.96mg_GA3/(g_Biomass穐), respectively. Using the coupling technology in the fermentation, the gibberellin production increased by 149.6% compared with controls without D-100 resin.

Key words: Gibberellic acid Macroporous resin Adsorption Coupling technology

收稿日期: 2012-05-03 出版日期: 2012-11-25

ZTFLH:

Q815

基金资助: 湖南省教育厅科学研究基金项目资助(11C1304)

通讯作者: 李忠海,电子信箱:lizh11@163.com E-mail: lizh11@163.com

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引用本文:

王卫, 李忠海, 黎继烈, 曾泊全. 赤霉素发酵与大孔树脂吸附过程耦合工艺优化[J]. 中国生物工程杂志, 2012, 32(11): 70-74.

WANG Wei, LI Zhong-hai, LI Ji-li, ZENG Bo-quan. Optimization of Fermentation Process for Gibberellic Acid Production Coupling with Adsorptive Extraction by Macroporous Resin. China Biotechnology, 2012, 32(11): 70-74.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I11/70

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