三个逆境相关的水稻启动子的克隆与瞬时表达分析

中国生物工程杂志

2012, Vol. 32

Issue (11): 29-34

研究报告

三个逆境相关的水稻启动子的克隆与瞬时表达分析

申秋硕, 陈凤, 叶青, 李涛, 陈信波, 张先文

湖南农业大学生物科学技术学院长沙 410128

Cloning and Transient Expression of the Three Stress-related Rice Promoters

SHEN Qiu-shuo, CHEN Feng, YE Qing, LI Tao, CHEN Xin-bo, ZHANG Xian-wen

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

全文: PDF(788 KB) HTML

摘要： 为了克隆和研究逆境诱导型水稻启动子的功能,根据前期工作所获得的高温条件下水稻幼穗的表达谱数据,结合对GEO数据库中有关水稻逆境芯片数据的检索与分析,选取了3个对高盐和高温显著响应的基因(Os11g0453900, Os09g0526600, Os05g0381400)。基于这3个基因ATG上游1.5kb的启动子序列的生物信息学分析,克隆了这3个启动子中包含有多个ABA、高温、高盐等逆境响应元件的DNA片段,分别命名为Rab16Dp、OsHsfB2cp和PM19p,并通过农杆菌介导转化烟草叶盘进行了瞬时表达分析。结果表明:高温、高盐对Rab16Dp和OsHsfB2cp启动子有较明显的诱导效应,而对PM19p的诱导效果不明显。实验为这3个启动子功能的进一步研究奠定了基础,也将为逆境诱导型水稻启动子的挖掘和鉴定提供重要线索。

关键词： 水稻; 启动子; 瞬时表达; 逆境诱导

Abstract: In order to clarify stress-inducible rice promoter function, the three heat- and salinity-induced genes (Os11g0453900, Os09g0526600, Os05g0381400) were picked out based on our previous microarray data of rice panicle and GEO database query. According to the bioinformatics analysis on the 1.5kb promoter sequences upstream of ATG, the 3 promoter fragments comprising specific cis-elements responsive to ABA, high temperature, high salinity were cloned, and named after Rab16Dp、OsHsfB2cp and PM19p, respectively. The results of Agrobacterium-mediated transient expression showed that Rab16Dp and OsHsfB2cp exhibited obvious activity to drive GUS gene expression under high temperature and salinity, but PM19p had no detectable activity under these conditions. The present work set the seal on the further research on the three promoters, and will provide important clues for the development of stress-inducible rice promoters.

Key words: Rice Promoter Transient expression Stress-inducible

收稿日期: 2012-08-13 出版日期: 2012-11-25

ZTFLH:

Q786

基金资助: 国家自然科学基金(31000125);湖南省教育厅青年基金(11B060)资助项目

通讯作者: 张先文,电子信箱:zhangxianwen@yahoo.com.cn E-mail: zhangxianwen@yahoo.com.cn

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引用本文:

申秋硕, 陈凤, 叶青, 李涛, 陈信波, 张先文. 三个逆境相关的水稻启动子的克隆与瞬时表达分析[J]. 中国生物工程杂志, 2012, 32(11): 29-34.

SHEN Qiu-shuo, CHEN Feng, YE Qing, LI Tao, CHEN Xin-bo, ZHANG Xian-wen. Cloning and Transient Expression of the Three Stress-related Rice Promoters. China Biotechnology, 2012, 32(11): 29-34.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I11/29

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