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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2012, Vol. 32 Issue (09): 55-60    
技术与方法     
MDCK细胞微载体悬浮培养放大工艺研究
龚迪, 易小萍, 张元兴
华东理工大学生物反应器工程国家重点实验室 上海 200237
A Study on Scale-up Process for Microcarrier Cultue of MDCK Cells Using Low Serum Medium
GONG Di, YI Xiao-ping, ZHANG Yuan-xing
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
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摘要： MDCK细胞对各种流感病毒具有高度敏感性,广泛应用于流感病毒的分离和疫苗制备。通过探索培养基中促进细胞贴壁的关键组分,并筛选水解物,开发了适合MDCK细胞生长的低血清培养基。发现钙、镁离子是细胞贴壁不可缺少的物质,麦麸水解物可以部分代替培养基中的血清。利用该低血清培养基,经过消化转移将MDCK细胞从5 L反应器放大至25 L反应器,微载体上细胞贴附均匀、生长旺盛,25 L反应器中培养48 h细胞密度可达30.5×105 cells/ml。研究结果为工业规模反应器微载体悬浮培养MDCK细胞生产流感病毒奠定了基础。
关键词： MDCK细胞;  微载体;  低血清培养基;  消化放大    
Abstract: Madin-Darby canine kidney (MDCK) cells have been widely used for influenza virus isolation and vaccine production since they are susceptible to infection with various influenza virus strains. To develop a low serum medium for MDCK cells, critical components that affect cell adhesion and favorable hydrolyzate were investigated and selected separately. Calcium and magnesium ions were found indispensable to cell adhesion, and serum could be partially replaced by wheat hydrolyzate in medium. MDCK cells were digested from microcarriers in 5 L bioreactor and then scaled up to 25 L bioreactor. The results showed that the low serum medium well supported the growth of MDCK cells with favorable cell distributions, and the viable cell density reached 30.5?105 cells/ml 48 h after inoculation in 25 L bioreactor. The work provided the necessary basis for large-scale production of avian influenza virus using microcarrier-based MDCK cell culture system.
Key words: Madin-Darbin canine kidney (MDCK) cell    Microcarrier    Low serum medium    Scale up by digestion
收稿日期: 2012-04-09 出版日期: 2012-09-25
ZTFLH:  Q813  
通讯作者: 易小萍     E-mail: xpyi@ecust.edu.cn
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引用本文:

龚迪, 易小萍, 张元兴. MDCK细胞微载体悬浮培养放大工艺研究[J]. 中国生物工程杂志, 2012, 32(09): 55-60.

GONG Di, YI Xiao-ping, ZHANG Yuan-xing. A Study on Scale-up Process for Microcarrier Cultue of MDCK Cells Using Low Serum Medium. China Biotechnology, 2012, 32(09): 55-60.

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https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I09/55

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