rhBMP-2对壳聚糖复合成骨细胞后的成骨活性影响

中国生物工程杂志

研究报告

rhBMP-2对壳聚糖复合成骨细胞后的成骨活性影响

左爱军梁东春王宝利郭刚张镜宇

天津医科大学内分泌研究所天津医科大学内分泌研究所天津医科大学内分泌研究所

Effect of rhBMP-2 on the Osteogenesis of Osteoblast Compounded Chitosan

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摘要： 将重组人骨形态发生蛋白2(rhBMP-2)与壳聚糖为主要基质的支架材料相复合，γ射线辐射灭菌后接种上原代培养的大鼠成骨细胞。体外培养3天后扫描电镜观察细胞与材料的黏附情况，可见成骨细胞紧密黏附于材料孔隙内并保持良好的生长状态。将接种有成骨细胞的复合材料植入裸鼠背部皮下，植入8周后X-ray摄片、组织学染色观察植入部位骨形成及支架材料降解情况。X-ray下可见与植入物大小、位置相符的高密度影，组织学染色证实材料的降解及孔隙内硬骨的生成。

Abstract: Recombinant human BMP-2 was compounded with chitosan/gelatin/hydroxyapatite(HCG) scaffold and the complex was sterilized by 60Co radiating. Osteoblast isolated from cranial bones of newborn rat was primary cultured and seeded onto the complexes. 3 days after culturing, scanning electron microscope(SEM) was applied to detect the compatibility of the cell with the complex. SEM showed osteoblast attached closely with the complex and grew well in its pores. Then the complexes with osteoblast modification were implanted into athymic nude mice subcutaneously. 8 weeks after implantation, X-ray photograph and histological observation were applied to detect the bone formation of the complexes. Under X-ray a high-density areas consistent with the shape of the implanted complex could be seen. Histological observation also proved there was bone formation in the interspace of the complex. A conclusion was drawn that rhBMP-2 compounded HCG scaffold had good osteogenesis ability in vivo.

收稿日期: 2005-09-15 出版日期: 2006-05-25

通讯作者: 梁东春

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引用本文:

左爱军,梁东春,王宝利,郭刚,张镜宇. rhBMP-2对壳聚糖复合成骨细胞后的成骨活性影响[J]. 中国生物工程杂志, .

. Effect of rhBMP-2 on the Osteogenesis of Osteoblast Compounded Chitosan. China Biotechnology, .

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I05/33

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