研究p21活化蛋白激酶2（p21-activated kinase 2，PAK2）在爪蟾卵母细胞成熟中的作用。利用特异性抑制PAK2活性的PAK2-N端（PAK2-N terminal,PAK2-NT）片段显微注射爪蟾卵母细胞。荧光显微镜下比较PAK2-NT mRNA注射组和未注射对照组卵母细胞胚泡破裂发生。共聚焦显微镜下，时间延迟摄影法观察两组卵母细胞胞质分裂过程中肌动蛋白和纺锤体的变化。与未注射PAK2-N端mRNA的对照组卵母细胞相比，注射组卵母细胞胚泡破裂发生无异常，但未见胞质分裂发生和极体形成。结果提示PAK2可能参与爪蟾卵母细胞胞质分裂过程。
Study the function of p21-activated kinase 2 (PAK2) in Xenopus oocyte maturation. Inject PAK2-N teminal (PAK2-NT) mRNA to Xenopus oocyte,and observe germinal vesicle breakdown by fluorescence microscope. To further observe the changes of F-actin and spindle, confocal microscopy with time-lapse was employed.Compared with the controls, injection of PAK2-NT mRNA caused no visible changes in oocyte morphology nor affected progesterone-induced germinal vesicle breakdown,but block cytokinesis and polar body formation. Maybe PAK2 involves in Xenopus oocytes maturation.
程大也,梁彬,李丰. p21活化蛋白激酶2在卵母细胞成熟中的作用[J]. 中国生物工程杂志, 2007, 27(6): 27-30.
. Function of p21-activated kinase 2 in oocyte maturation. China Biotechnology, 2007, 27(6): 27-30.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I6/27
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