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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2011, Vol. 31 Issue (03): 97-100    
综述     
反向遗传学在呼吸道合胞病毒减毒活疫苗研究中的应用
付远辉1, 何金生1, 洪涛1,2
1. 北京交通大学生命科学与生物工程研究院 北京 100044;
2. 中国疾病预防控制中心病毒病预防控制所院士实验室 北京 100052
The Application of Reverse Genetics to the Live-attenuated RSV Vaccine
FU Yuan-hui1, HE Jin-sheng1, HONG Tao1,2
1. College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China;
2. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
 全文: PDF(344 KB)   HTML
摘要:

人呼吸道合胞病毒(human respiratory syncytial virus, RSV)是引起婴幼儿下呼吸道感染的最重要的病毒病原,减毒RSV活疫苗能模拟自然感染充分活化机体固有免疫系统,并诱导产生体液免疫和细胞免疫,不会产生疾病增强作用,经黏膜途径应用,能突破母传抗体的干扰,因而受到广泛关注,反向遗传学(reverse genetics)在减轻野生型RSV毒力和增强其免疫原性等方面具有传统减毒技术不可比拟的优势,所以综述了反向遗传学在RSV减毒活疫苗研究中的应用。

关键词: 呼吸道合胞病毒反向遗传学减毒活疫苗    
Abstract:

Human respiratory syncytial virus (RSV) is the leading viral agent of serious respiratory disease in infants and young children worldwide, infecting nearly all children one or more times by age 2. An intranasal live-attenuated RSV vaccine mimics natural infection and presumably induces the same broad array of local and systemic immunity, including innate and cell-mediated immunity as well as mucosal and serum antibodies. The intranasal route is albe to escapes the immunosuppressive effects of maternal serum antibodies. Additionally, the immune-mediated enhanced disease, induced by nonreplicating killed RSV vaccine, is not associated with attennated RSV vaccine candidates. A number of live-attenuated RSV vaccine candidates have been developed by conventional methods. However, there are many advantages for developing attenuated strains by reverse genetics.The application of reverse genetics to the live-attenuated RSV vaccine is reviewed.

Key words: Respiratory syncytial virus    Reverse genetics    Live-attenuated vaccine
收稿日期: 2010-09-30 出版日期: 2011-04-01
ZTFLH:  Q939.44  
基金资助:

国家自然科学基金(30671965,30972604)、北京市自然科学基金(7092053)、中国博士后科学基金(20100470196)资助项目

通讯作者: 何金生     E-mail: jshhe@bjtu.edu.cn
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付远辉, 何金生, 洪涛. 反向遗传学在呼吸道合胞病毒减毒活疫苗研究中的应用[J]. 中国生物工程杂志, 2011, 31(03): 97-100.

FU Yuan-hui, HE Jin-sheng, HONG Tao. The Application of Reverse Genetics to the Live-attenuated RSV Vaccine. China Biotechnology, 2011, 31(03): 97-100.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I03/97

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