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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2011, Vol. 31 Issue (03): 91-96    
综述     
体外核酸快速扩增技术的发展和不断创新
吕蓓1, 程海荣2, 严庆丰4, 黄震巨3, 李轶女1, 罗达5, 沈桂芳1, 张志芳1, 邓子新2, 林敏1, 程奇1
1. 中国农业科学院生物技术研究所 北京 100083;
2. 上海交通大学生命科学技术学院 微生物代谢教育部重点实验室 上海 200240;
3. 浙江泰晶生物科技有限公司 宁波 315100;
4. 浙江大学生命科学院 杭州 310058;
5. 中山大学生命科学院 广州 510275
The Development and Recent Improvements of in Vitro Nucleic Acid Amplification Technology
LV Bei1, CHENG Hai-rong2, YAN Qing-feng4, HUANG Zhen-ju3, LI Yi-nv1, LUO Da5, SHEN Gui-fang1, ZHANG Zhi-fang1, DENG Zi-xin2, LIN Min1, CHENG Qi1
1. Biotechnology Research Institure,Chinese Academy of Agricultural Sciences,Beijing 100081, China;
2. Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;
3. Zhejiang Taijing Biotechnology Limited, Ningbo 315100, China;
4. College of Life Science,Zhejiang University, Hangzhou 310058, China;
5. College of Life Science, Sun Yat-sen University, Guangzhou 510275, China
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摘要:

利用聚合酶链式反应(PCR)进行的核酸体外扩增是1983年开始发展起来的一项革命性技术,目前已被广泛运用于现代化的农业和医学以及食品工业等领域,特别是在人类认知基因和基因组的过程中,体外核酸扩增技术做出了卓越的贡献。最初,体外核酸扩增技术主要是利用耐高温的DNA聚合酶(Taq酶),这样就使核酸的体外扩增反应可以在热循环中进行。但因需要使用昂贵的设备和消耗大量的电力,其成本和应用范围都受到一定的限制。之后,恒温体外核酸扩增悄然兴起,这改变了传统扩增技术的局限性,使核酸的体外扩增更加简单和方便。重组酶介导扩增(RAA)法是一种最新型的恒温体外核酸扩增技术,该系统的显著优点在于它在常温下就能实现DNA解链并快速扩增(15~30min完成),反应快速、专一性好、灵敏度高,还可用于定时定量的结果分析。

关键词: 重组酶介导核酸体外扩增恒温    
Abstract:

Rapid nucleic acid amplification in vitro is a revolutionary technology developed since 1983. It has been applied widely in modern agriculture, medical and food science etc.. Such technology has provided a great tool for gene manipulation and functional genomics. In vitro nucleic acid amplification is usually carried out by a thermal stable enzyme, such as Taq polymerase, and requires thermal cycle equipment. The invention of isothermal amplification has improved the traditional PCR method and in some way simplified the reaction procedure. Recombinase-aid amplification (RAA) was introduced. RAA is the latest isothermal amplification technology which may be carried out under the room temperatures. The distinctive merit of RAA is mimicking in vivo T4-phage DNA amplification complex and the whole reaction is super fast and specific. Moreover, it can also be used for digital real time quantitative analysis.

Key words: Recombinase-aid    Nucleic acid amplification in vitro    Isothermal
收稿日期: 2010-11-11 出版日期: 2011-04-01
ZTFLH:  Q784  
通讯作者: 程奇     E-mail: chengqi@caas.net.cn
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吕蓓
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罗达
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邓子新
林敏
程奇

引用本文:

吕蓓, 程海荣, 严庆丰, 黄震巨, 李轶女, 罗达, 沈桂芳, 张志芳, 邓子新, 林敏, 程奇. 体外核酸快速扩增技术的发展和不断创新[J]. 中国生物工程杂志, 2011, 31(03): 91-96.

LV Bei, CHENG Hai-rong, YAN Qing-feng, HUANG Zhen-ju, LI Yi-nv, LUO Da, SHEN Gui-fang, ZHANG Zhi-fang, DENG Zi-xin, LIN Min, CHENG Qi. The Development and Recent Improvements of in Vitro Nucleic Acid Amplification Technology. China Biotechnology, 2011, 31(03): 91-96.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I03/91

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