中国生物工程杂志

In order to investigate the effects of Osx on Osteoblast proliferation and differentiation, recombinant Osx-expressing adenovirus were constructed. Osx-coding sequence was cloned into adenovirus vector, which was transfected to 293A for packaging, primarily cultured mouse osteoblasts were infected with the virus, alizarin red staining was used for mineralization analysis, realtime quantitative RT-PCR was used for differentiation assay, cell cycle was checked using FACS. Results show that ① Virus titer was about 2×109/ml，most optimal multiplicity of infection was 50, ② Overexpression of Osx did not promote osteoblast mineralization, ③ 1d,3d and 6d after overexpression of Osx upregulated the transcription level of Osteocalcin, BSP and collagen Ⅰsignificantly(p<0.01), ④ Osx promoted osteoblast proliferation(p<0.01). Overexpression of Osx in osteoblast promotes proliferation of osteoblast, and regulates osteoblast differentiation to a certain degree.