Please wait a minute...

中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
研究简报     
水牛SRY基因的克隆及序列分析
付强 张明 卢克焕 秦文松 郑海英
广西大学动物繁殖研究所 广西亚热带生物资源保护利用重点实验室 广西亚热带生物资源保护利用重点实验室
Cloning and characterization of swamp Buffalo SRY gene
 全文: PDF  HTML
摘要: 根据荷斯坦牛SRY基因设计一对引物,采用聚合酶链式反应(PCR)技术,以中国沼泽型水牛(Swamp Buffalo)基因组DNA为模板,扩增得到SRY(Sex Deterimation region of Y chromosome)全序列约2005bp,其中1-504bp为5’启动子区,1196-2005bp为3’侧翼序列,在505-1195bp为SRY的外显子,编码229个氨基酸。在SRY HMG box区域设计探针,用地高辛标记后分别与雄性、雌性水牛基因组DNA进行Southern 杂交,结果显示该段序列只在雄性DNA样本中有杂交信号,证明SRY基因为雄性特异。BLAST比对结果显示与牛属动物SRY基因的同源性为96%,其中SRY基因HMG box区域同源性高达99%,说明SRY基因具有高度的进化保守性
Abstract: The SRY gene from Buffalo (Bubalus bubalis) genome was amplified by the polymerase chain reaction (PCR) with primers based on the sequence of Hostein SRY gene. The amplified fragment was 2005 bp include 5’ UTR(1~504bp) and 3’UTR(1196-2005bp). And The amplified fragment was cloned and sequenced. Sequence analysis showed that the coding region of SRY gene(505~1195bp) from Buffalo was highly homologous with those of other Bovine counterpart genes (96% homology), especially in the HMG box region (99%homology). It was found that there were only signal on male Buffalo genome on Southern blot,which indicate SRY gene are highly conservative on evolves.
收稿日期: 2006-02-23 出版日期: 2006-07-25
通讯作者: 付强   
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  
张明
秦文松
郑海英
卢克焕
付强

引用本文:

付强,张明,卢克焕,秦文松,郑海英. 水牛SRY基因的克隆及序列分析[J]. 中国生物工程杂志, .

. Cloning and characterization of swamp Buffalo SRY gene. China Biotechnology, .

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I07/69

No related articles found!