Please wait a minute...

中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2010, Vol. 30 Issue (05): 76-80    
研究报告     
耐碱的偶氮染料脱色菌筛选及其特性的研究
张胜琴1,陈必强2,杨光3**,李春4,曹竹安2**
1.石河子大学化学化工学院 石河子 832003
2.清华大学化工系生物化工研究所 北京 100084
3.中国科学院天津工业技术生物研究所 天津 300308
4.北京理工大学生命学院 北京 100081
Screening and Characteristics of Azo dye Decoloring Alkali-resistant Bacteria
ZHANG Sheng-qin1,CHEN Bi-qiang2,YANG Guang3,LI Chun4,CAO Zhu-an2
1.College of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China
2.Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
3.Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
4.School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
 全文: PDF(541 KB)   HTML
摘要：

从浙江某污水处理厂的活性污泥中筛选出若干株在高pH条件下对偶氮染料酸性大红GR有脱色能力的菌株，经脱色验证得到一株具有高效脱色活性的菌株Z1，经鉴定为巴斯德葡萄球菌(Staphylococcus pasteuri)，并对此菌株的脱色特性进行了初步研究。结果表明，在厌氧条件下，Z1在pH7~12，40h对50mg/L的酸性大红GR脱色率均可达90%以上。该菌株对染料有较强的耐受力，在酸性大红GR浓度为300mg/L时，48h的脱色率仍可达93%。此外，该菌株能够对多种偶氮染料脱色，具有较好的脱色广谱性，有望应用于处理工业废水中的偶氮染料。
关键词： 偶氮染料;  巴斯德葡萄球菌;  耐碱菌;  广谱性    
Abstract:

Studies were carried out to isolate Acid Scarlet GR resistant and decolored bacteria from sludge of a wastewater treatment plant, Zhejiang Province. Through dyeing decoloring tests, one strain, named Z1 was found to have a better decolorization performance at the high pH 12 and identified as Staphylococcus pasteuri by 16SrDNA gene sequence analysis. A strain named Z1 was screened through dye decolorization test and was identified as Staphylococcus pasteuri by 16SrDNA gene sequence analysis. And then the decolorization properties of Z1 were investigated. The results showed that the decolorization rate of 50mg/L Acid Scarlet GR could exceed 90% within 40h under anaerobic conditions at the range of pH 7~12, and the decolorization rate of 300mg/L Acid Scarlet GR even reached 93% within 48h. Moreover, the strain could effectively degrade other azo dyes with broad spectrum and could be applied for removing the azo dyes from the industrial wastewater.
Key words: azo dye    Staphylococcus pasteuri    Alkali-resistant bacteria    broad-spectru
收稿日期: 2010-03-10 出版日期: 2010-05-25
基金资助:

国家“863”计划（2007AA021306）资助项目
通讯作者: 曹竹安     E-mail: yang_g@tib.cas.cn
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
张胜琴
陈必强
杨光
李春
曹竹安

引用本文:

张胜琴 陈必强 杨光 李春 曹竹安. 耐碱的偶氮染料脱色菌筛选及其特性的研究[J]. 中国生物工程杂志, 2010, 30(05): 76-80.

ZHANG Qing-Qin, CHEN Bi-Jiang, YANG Guang, LI Chun, CAO Zhu-An. Screening and Characteristics of Azo dye Decoloring Alkali-resistant Bacteria. China Biotechnology, 2010, 30(05): 76-80.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I05/76

［1］ 赵雪, 何瑾馨, 展义臻. 印染废水处理技术的研究进展. 化学工业与工程技术, 2009, 30(2): 3843. Zhao X, He J X, Zhan Y Z. Journal of Chemical Industry & Engineering, 2009, 30（2）: 3843. 
［2］ Saratale R G, Saratale G D. Decolorization and biodegradation of textile dye Navy blue HER by Trichosporon beigelii NCIM3326. Journal of Hazardous Materials, 2009, 166: 14211428． 
［3］ 王慧, 周月霞, 柏仕杰,等. 染料废水生物法处理技术的研究进展. 厦门大学学报, 2008, 47(2): 286290. Wang H, Zhou Y X, Bo S J, et al. Journal of Xiamen University(Natural Science), 2008, 47（2）: 286290. 
［4］ 张波, 白云峰, 朱斌.两株细菌对偶氮染料活性艳红X3B脱色的研究.山西大学学报(自然科学版),1998,21(1):7779. Zhang B, Bai Y F, Zhu B. Journalof Xiamen University(Natural Science), 1998,21(1):7779. 
［5］ 范琼, 张学亮, 张弦. 橘子皮对水中亚甲蓝的吸附性能研究. 中国生物工程杂志, 2007, 27(5): 8589. Fan Q, Zhang X L, Zhang X. China Biotechnology, 2007, 27(5): 8589. 
［6］ Deng D Y, Guo J. Decolorization of anthraquinone, triphenylmethane and azo dyes by a new isolated Bacillus cereus strain DC11. International Biodeterioration & Biodegradation, 2008, 62: 263269. 
［7］ Yang Q X, Yang M. Decolorization of synthetic dyes and production of manganesedependent peroxidase by new fungal isolates. Biotechnology Letters, 2003, 25: 709713. 
［8］ 章燕芳, 李华钟, 华兆哲，等. 木质素过氧化物酶和锰过氧化物酶对染料脱色的性能比较. 环境科学研究, 2002, 15(5): 1721. Zhang Y F, Li H Z, Hua Z Z. Environmental Science and Research, 2002, 15(5): 1721. 
［9］ Acuner E, Dilek F B. Treatment of tectilon yellow 2G by Chlorella vulgaris. Process Biochemistry, 2004, 39 (5): 623631. 
［10］ Yogesh M. Kolekar, Shrikant P. Pawar, Kachru R. Gawai. et.al. Decolorization and degradation of Disperse Blue 79 and Acid Orange 10,by Bacillus fusiformis KMK5 isolated from the textile dye contaminated soil. Bioresource Technology, 2008, 99: 89999003. 
［11］ Xu M Y, Guo J, Sun G P. Biodegradation of textile azo dye by Shewanella decolorationis S12 under microaerophilic conditions. Appl Microbiol Biotechnol, 2007,76: 719726. 
［12］ 徐向阳, 张明洲, 俞秀娥. 高效脱色菌的特性及其在染化废水厌氧处理中的生物强化作用. 中国沼气, 2001, 19(2): 37. Xu X Y, Zhang M Y, Yu X E. China Biogas, 2001, 19(2): 37. 
［13］ 杨东虎, 阚振荣. 偶氮染料直接大红GBE 脱色细菌的筛选及研究. 环境污染治理技术与设备, 2002, 3(12): 5153. Yang D H, Que Z R. Techniques and Equipment for Environmental Pollution Control, 2002, 3(12): 5153. 
［14］ 李慧蓉, 李华兵, 李文琼，等. 黄孢原毛平革菌对6种染料的脱色降解. 环境科学研究, 1999, 12(3): 1417. Li H R, Li H B, Li W Q,et al. Environmental Science and Research, 1999, 12(3): 1417. 
［15］ Spadaro J T, Gold M H, Ren V. Degradation of azo dyes by the lignindegrading fungus phanerochaete chrysosporium. Appl Environ Microbiol,1992,58(8):21972401. 
［16］ 宴再生,王世和,吴慧芳.不同环境条件下偶氮染料酸性大红GR 的生物降解性能.城市环境与城市生态, 2008,21(5):14. Yan Z S, Wang S H, Wu H F. Urban Environment & Urban Ecology, 2008,21(5):14. 
［17］ Chang J S, Chou C, Lin P J, et al. Kinetic characteristics of bacterial azo dye decolorization by pseudomonas luteola. Water Research, 2001, 35(12): 28412850. 
［18］ 吴赞敏, 吕彤, 翁亮,等. 生物菌对活性染料的脱色研究. 纺织学报, 2006,27(5):4548. Wu Z M, Lv T, Weng L. Journal of Textile, 2006,27(5): 4548. 
［19］ 张金平, 阚振荣, 梁利华. 脱色细菌的分离和对偶氮染料的脱色研究. 生物学杂志, 2006,23(2): 3538. Zhang J P, Que Z R, Liang L H. Journal of Biology, 2006,23(2): 3538. 
［20］ 付永胜, 魏剑斌, 李湘梅. 活性红PBL脱色菌的筛选及其特性. 西南交通大学学报, 2005,40(4):561564. Fu Y S, Wei J B, Li X M. Journal of Southwest Jiaotong University, 2005,40(4):561564. 
［21］ Shailesh R. Dave, Riddhi H. Dave. Isolation and characterization of Bacillus thuringiensis for Acid red 119 dye decolourisation. Bioresource Technology, 2009, 100: 249253.
No related articles found!
主管：中国科学院 　主办：中国科学院文献情报中心 　中国生物技术发展中心 　中国生物工程学会