目的：采用反应器技术以细胞为基质培养流感病毒，并探索其纯化工艺。 方法：采用5L反应器无血清条件下以Vero细胞为基质培养流感病毒，病毒收获后，经灭活、澄清，采用阴离子柱去除宿主DNA，亲和柱浓缩流感病毒，最后采用分子筛三步层析法进行纯化。结果：整个纯化工艺HA回收率达102%，蛋白降低率为95.3%，宿主蛋白降低率为99.77%，DNA的降低率为99.99%。结论 本研究成功建立了一种细胞流感病毒的纯化工艺。
Objective Establish mass-scale purification technology of cell-derived influenza virus. Methods A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion This research provides a high effective purification technology for cell-derived influenza virus.
其它项目基金
何春艳,陈则,吴书军,唐丽丽,秦洁. Vero 细胞流感病毒的大规模培养和纯化[J]. 中国生物工程杂志, 2008, 28(11): 20-24.
https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I11/20
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