以三尖杉幼茎为培养材料，诱导出愈伤组织，并分析了不同的生长素组合、温度、糖浓度、接种量、活性炭等因素对愈伤组织诱导和生长的影响，结果表明，NAA激素组合比2，4-D更适合三尖杉愈伤组织的诱导，在25±1℃、3%蔗糖、0.75%琼脂、完全黑暗的条件下愈伤组织诱导率可达90%以上。在100ml的容量瓶中，2.0g的接种量适合三尖杉愈伤组织的继代培养，同时，一定量的活性炭可以适当改善愈伤组织的褐化状况。
Abstract : callus were induced from cephalotaxue fortunei Hook.f, the effect of hormone, sugar concentration, temperature and quantity of inoculation on the inductivity were studied. under the condition of 3% sugar concentration, 0.75% agar concentration, and 25±1℃,the rate of callus inductivity reached to 90%.the callus inoculation of 2.0g accelerate the growth of callus in a flask 100ml.
许金光,刘长江,张卫,岳喜庆,金美芳. 三尖杉愈伤组织培养的研究[J]. 中国生物工程杂志, 2006, 26(0): 57-60.
. Study in callus culture of cephalotaxue fortunei Hook.f. China Biotechnology, 2006, 26(0): 57-60.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/57
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