本文比较了胶体金免疫层析测试、DIA(斑点免疫结合技术)与PCR三种检测柑橘溃疡病菌的常用方法的灵敏度、特异性及实际检测应用等方面的差异。采用柠檬酸三钠还原法制备胶体金颗粒,标记柑橘溃疡病菌多克隆抗体,制成免疫速测卡。用于柑桔溃疡病菌现场快速检测,为基层和田间快速筛检提供了新的诊断方法。
The sensitivity, specificity and application and expenses among the gold immunochromatography assay (CGIA), DIA (Dot Immunobinding Assay) and PCR assay have been compared. Use trisodium citric acid deoxidization to prepare colloidal gold, and then it was coupled with rabbit polyclonal antibody of Xac04 pathogen, to produce the fast reagent strip of CGIA. A new useful method for rapid screening assay of CBCD in field was developed.
李平,王中康,谭万忠,夏玉先,殷幼平. 利用胶体金免疫层析法、斑点免疫法与PCR技术检测[J]. 中国生物工程杂志, 2006, 26(0): 79-81.
. Application of Colloidal Gold Immunochromatography Assay, DIA and PCR for detection of CBCD. China Biotechnology, 2006, 26(0): 79-81.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/79
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