中国生物工程杂志

A 501bp sequence of Sus scrofa IFN-αgene and Susscrota domestica of changbai IFN-αgene was amplified by normal polymerase chain reaction (PCR) from their genome whose homology are more than 98.4% ，compared with nucleotide sequence published on NCBI .Referring to Sus scrofa IFN-αgene sequence,a new sequence was synthesized after codon bias and mRNA structure optimizaed .Then ,this sequence was inserted in pWL vector and the recombinant plasmids were transformed into the E.Coli DH-5α.After fermentation,the expression level of recombinant protein are 25% by SDS-PAGE analysis.The biologic activity was 4.45×106 IU/mg by VSV-WISH system analysis after protein refolding and purification.