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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2011, Vol. 31 Issue (06): 135-141    
综述     
微生物发酵法生产L-色氨酸的代谢工程研究
赵志军1,2, 陈晟1,2, 吴丹1,2, 吴敬1,2, 陈坚1,2
1. 江南大学食品科学与技术国家重点实验室 无锡 214122;
2. 江南大学生物工程学院工业生物技术教育部重点实验室 无锡 214122
Metabolic Engineering of L-tryptophan via Microbiological Fermentation
ZHAO Zhi-jun1,2, CHEN Sheng1,2, WU Dan1,2, WU Jing1,2, CHEN Jian1,2
1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2. School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi 214122, China
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摘要:

L-色氨酸作为人体内的一种必需氨基酸,广泛应用于医药、食品与饲料等行业。工业上采用的色氨酸生产方法有化学合成法、转化法及微生物发酵法。近年来,随着代谢工程在色氨酸菌种选育中的成功运用,微生物发酵法逐渐成为主要的色氨酸生产方法。系统综述了微生物发酵法生产色氨酸所涉及的代谢工程策略,包括生物合成色氨酸的代谢调控机制以及途径改造的措施和效果,此外,还探讨了L-色氨酸未来的发展前景。

关键词: L-色氨酸代谢工程微生物发酵法    
Abstract:

L-tryptophan (L-Trp) is widely used in food, animal feed and pharmaceutical industries as an essential amino acid for humans. Chemical synthesis, enzymatic/microbial conversion and microbial fermentation are the methods for industrial production of L-Trp. Recently, with successful application of metabolic engineering in strain improvement, microbial fermentation gradually becomes the major method of L-Trp production. The strategy of metabolic engineering for improving L-Trp production is reviewed, involving the regulatory mechanism and genetic modification of L-Trp biosynthesis. Furthermore, the prospect of L-Trp production is also discussed.

Key words: L-tryptophan    Metabolic engineering    Microbial fermentation
收稿日期: 2010-12-20 出版日期: 2011-06-28
ZTFLH:  Q819  
基金资助:

国家"863"计划 (2009AA02Z204)、国家杰出青年基金(20625619)资助项目

通讯作者: 吴敬     E-mail: jingwu@jiangnan.edu.cn
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引用本文:

赵志军, 陈晟, 吴丹, 吴敬, 陈坚. 微生物发酵法生产L-色氨酸的代谢工程研究[J]. 中国生物工程杂志, 2011, 31(06): 135-141.

ZHAO Zhi-jun, CHEN Sheng, WU Dan, WU Jing, CHEN Jian. Metabolic Engineering of L-tryptophan via Microbiological Fermentation. China Biotechnology, 2011, 31(06): 135-141.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I06/135

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