多基因表达系统研究进展

中国生物工程杂志

2011, Vol. 31

Issue (06): 116-123

综述

多基因表达系统研究进展

楚素霞^1,2, 姚伦广², 邢延豪¹, 周延清¹

1. 河南师范大学生命科学学院新乡 453007;
2. 河南省伏牛山昆虫生物学重点实验室南阳师范学院中英联合实验室南阳 473061

Advances on the Study of Multi-gene Expression System

CHU Su-xia^1,2, YAO Lun-guang², XING Yan-hao¹, ZHOU Yan-qing¹

1. School of Life Sciences, He Nan Normal University, Xinxiang 453007,China;
2. He Nan Provincial Key Laboratory of Funiu Mountain Insect Biology, China-UK, Nan Yang Normal University-Rothamsted Research Joint Laboratory of Insect Biology, Nanyang Normal University, Nan yang 473061, China

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摘要：

细胞中大多数蛋白质以亚基形式与其他蛋白装配成蛋白复合体而发挥功能。大分子蛋白复合物的结构研究和功能分析在后基因组时代成为热点,如何高效地获得多蛋白复合物是研究其功能和结构的前提。利用基因工程技术实现多个蛋白亚基在同一宿主细胞内共表达并装配成复合体是获得多蛋白复合物的有效手段。多基因表达系统在基础和应用研究中正起到越来越重要作用。多基因表达系统研究发展迅速,尤其在近5年内取得较大进步。对各种多基因表达系统进行了总结。同时介绍了不同多基因表达系统的构建策略和构建方法,并对最新的多基因表达载体构建和多基因表达系统的初步应用进行了介绍。多基因表达系统将在作物改良、疫苗制备、基因治疗和药物开发方面发挥更大作用。

关键词： 蛋白复合物; 多基因表达系统; 表达盒; 归位酶; 同尾酶技术

Abstract:

Most of cellular proteins work as the subunits of protein complexes which are the basic functional unit in cell. The structure research and functional analysis of macromolecule complexes became popular in the post-genomic era. One major challenge is to produce these complexes in sufficient amounts for biochemical and structural research. One efficient way which named mutigene expression system is used widely to obtain the protein complexes by co-expressing subunits simultaneously in host cells. Multigene expression system is becoming more and more important on both basic and application researches. The studies on multigene expression system have been developed quickly, especially in the recent five years. The studies on multigene expression system, which provided the knowledge on it's characters and usages were summarized. The detailed construction methods of different kinds of multigene expression vectors, especially the newest vectors, which will be helpful to choose the most efficient system for protein complexes studies were introduced. The primary applications of the multigene expression system are also introduced.

Key words: Protein complex Multigene expression system Expression cassette Homing endonuclease Isocaudamer technique

收稿日期: 2010-12-21 出版日期: 2011-06-28

ZTFLH:

Q786

基金资助:

国家自然科学基金(81072494)、河南省科技厅重点攻关项目(102102310090)资助项目

通讯作者: 姚伦广 E-mail: lunguangyao@163.com

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引用本文:

楚素霞, 姚伦广, 邢延豪, 周延清. 多基因表达系统研究进展[J]. 中国生物工程杂志, 2011, 31(06): 116-123.

CHU Su-xia, YAO Lun-guang, XING Yan-hao, ZHOU Yan-qing. Advances on the Study of Multi-gene Expression System. China Biotechnology, 2011, 31(06): 116-123.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I06/116

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