响应面法优化自养小球藻产生物柴油油脂

doi:Q819

中国生物工程杂志

2010, Vol. 30

Issue (08): 106-111 DOI: Q819

技术与方法

响应面法优化自养小球藻产生物柴油油脂

郑洪立¹,高振^1**,黄和^1,2,纪晓俊¹,白跃华³,李文琦³

1.南京工业大学生物与制药工程学院南京 210009
2.材料化学工程国家重点实验室南京 210009
3.中国石油天然气股份有限公司石油化工研究院北京 100029

Optimization of Autotrophic Cultivation of Lipids Production for Biodiesel by Chlorella vulgaris with Response Surface Methodology

ZHENG Hong-li¹,GAO Zhen¹,HUANG He^1,2,JI Xiao-jun¹,BAI Yue-hua³,LI Wen-qi³

1.College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
2.State Key Laboratory of MaterialsOriented Chemical Engineering, Nanjing 210009, China
3.Petroleum Research Institute, China National Petroleum Corporation, Beijing 100029, China

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摘要：

利用响应面法对小球藻（Chlorella vulgaris）在2 L气升式生物反应器中对自养产生物柴油油脂的培养条件进行了优化。首先用Plackett-Burman方法对10 个相关影响因素的效应进行评价并筛选出对产油有显著影响的3 个因素：KNO3浓度、温度和CO2浓度；再用最陡爬坡实验逼近最大产油区域；最后由中心组合实验及响应面分析确定了影响产油主要因素的最佳条件为：KNO3浓度0.31g/L,温度26.5 ℃，CO2浓度6.80%，最高产油量达到0.42 g/L，比优化前提高了近2 倍。优化后,在10 L气升式生物反应器中进行了扩大培养。

关键词： 小球藻; 培养条件; 响应面法; 生物柴油; 油脂

Abstract:

Response surface methodology was used to optimize the autotrophic cultivation conditions for lipids production by Chlorella vulgaris in a 2-L airlift photobioreactor. In the first optimization step, a Plackett-Burman design was used to evaluate the influence of ten related factors and it was found out that KNO3 concentration, temperature and CO2 concentration influenced lipids production significantly. Subsequently, the path of steepest ascent was used to approach the optimal region of the cultivation conditions. In the third step, KNO3 concentration, temperature and CO2 concentration were further optimized using central composite designs and response surface analysis and the optimum conditions were that KN03 concentration was 0.31 g/L, temperature was 26.5 ℃ and CO2 concentration was 6.80%. Under optimum conditions, the lipid yield was 0.42 g/L and it was increased by two times than that under the conditions before optimized. Chlorella vulgaris was cultured in a 10-L airlift photobioreactor under optimum conditions.

Key words: Chlorella vulgaris Cultivation conditions Response surface Methodology BiodieselLipids

收稿日期: 2010-04-21 出版日期: 2010-08-25

基金资助:

国家自然科学基金(20936002)、国家“973”计划(2007CB707805, 2009CB724700)、江苏省六大人才高峰项目 (2008)、江苏省高校科研成果产业化推进项目(2009)资助项目

通讯作者: 高振 E-mail: gaozhen@njut.edu.cn

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引用本文:

郑洪立高振黄和纪晓俊白跃华李文琦. 响应面法优化自养小球藻产生物柴油油脂[J]. 中国生物工程杂志, 2010, 30(08): 106-111.

ZHENG Hong-Li, GAO Zhen, HUANG He, JI Xiao-Dun, BAI Ti-Hua, LI Wen-Qi. Optimization of Autotrophic Cultivation of Lipids Production for Biodiesel by Chlorella vulgaris with Response Surface Methodology. China Biotechnology, 2010, 30(08): 106-111.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/Q819 或 https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I08/106

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