法夫酵母高产虾青素的选育及发酵条件的优化

中国生物工程杂志

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法夫酵母高产虾青素的选育及发酵条件的优化

谢虹周元元胡卫成梁建生

扬州大学生物科学与技术学院信阳师范学院生命科学学院信阳师范学院生命科学学院

Mutagenesis Screening of Astaxanthin-producing Phaffia rhodozyma and Fermentation Condition Optimization

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摘要： 对法夫酵母原始菌株进行了紫外线－氯化锂复合诱变，获得了一株遗传性状稳定、在25℃条件下虾青素高产的菌株UL-40。其类胡萝卜素产量为7.10mg/L，经HPLC测定，其虾青素产量为643.97μg/g，比出发菌株提高了249.87%。利用SAS软件中的Plackett-Burman设计对发酵温度、初始pH值以及发酵培养基的八种组分进行优化组合，从中选出发酵温度、初始pH值和Corn steep liquor浓度为重要因素，通过响应面分析法建立了模型并从该模型中计算出在发酵温度为16.78℃、初始pH为4.73和CSL浓度为7.06mg/L时预测的最大响应值为3.9407mg/L，经实验证实此点的实测值为3.9261mg/L，证明模型是有效的并且存在极大值点。采用优化后的发酵条件及发酵培养基，法夫酵母生产虾青素的产量较原始发酵培养基提高了20.4%。

Abstract: The original strain was treated with UV combining LiCl. The result showed that strain UL-40 has a good stability and higher capacity to synthesize astaxanthin under 25℃. The total carotenoid yield was 7.10mg/L. HPLC analysis showed that the maximal yield of astaxanthin of the selected strain had reached 643.97μg/g, which was 249.87% higher than that of the initial strain. The fermentation temperature, initial pH of culture solution and 6 kinds of substrate were optimized by Plackett-Burman design and Response Surface Analysis provided by SAS software. The temperature, initial pH and corn steep liquor were chosen as the major factors from all the 8 variables, a regression model equation was obtained after regression analysis performed on the data from RSA experiment. The optimal conditions were predictted to be 16.78℃, pH4.73 and 7.06 mg/L, respectively, for the maximum carotenoid production. Under these conditions, the theroretical maximal yield of carotenoid was 3.9407 mg/L. The model was validated experimentally and the practical maximal yield of carotenoid was about 3.9261mg/L. The carotenoid production was increased by 20.4% when P. rhodozyma was cultivated under the optimal conditions as compared with the control.

收稿日期: 2006-06-26 出版日期: 2006-12-25

通讯作者: 谢虹

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引用本文:

谢虹,周元元,胡卫成,梁建生. 法夫酵母高产虾青素的选育及发酵条件的优化[J]. 中国生物工程杂志, .

. Mutagenesis Screening of Astaxanthin-producing Phaffia rhodozyma and Fermentation Condition Optimization. China Biotechnology, .

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I12/68

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