中国生物工程杂志

An important intermediate of deoxyadenosylcobalamin biosynthesis by anaerobic pathway of Propionibacterium freudenreichii was separated from the broth in this paper. It has been qualitatively analyzed by spectrum and ESI MS-GC analysis and further determined as a key intermediate of deoxyadenosylcobalamin: adenosylcobinamide which can be transformed to deoxyadenosylcobalamin when infused 5,6-dimethylbenzimidazole (DMB) to the fermentation medium. It is hope that the intermediate could be a key factor which guides the infusion of DMB and further optimize the fermentation process.