综述了纳豆激酶的分离纯化技术的研究现状和发展趋势。通过对常规分离技术的分析,重点讨论了集成化分离技术的应用及其优势,包括集成化双水相分配技术、扩张床吸附技术以及耐盐性混合模式吸附技术等分离方法。并指出集成化分离技术在生产纳豆激酶以及其他活性蛋白方面,具有广阔的应用前景。
The progress in separation and purification technology of nattokinase is reviewed. Based on the analyze of normal purification technology, the novel technologies about the integration of bioseparation processes are introduced, such as aqueous two-phase partitioning integrated with affinity ligand, expanded bed adsorption, and mixed-mode adsorption technology. In general, integration of bioseparation processes provides a good way to improve and optimize the whole separation process of nattokinase and other proteins.
苟金霞,高栋. 纳豆激酶集成化分离技术[J]. 中国生物工程杂志, 2008, 28(1): 119-123.
. Novel progress in separation and purification technology of nattokinase. China Biotechnology, 2008, 28(1): 119-123.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I1/119
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