昆虫防御素是防御素的一种,也是近年来发现的一组新型抗菌活性肽。它是一类富含精氨酸的阳离子小肽,对革兰氏阳性细菌能产生较强的杀伤作用。 根据GenBank(AY260152)提供的家蝇(houseflies,Musca domestica)防御素(defensin)基因,选择毕赤酵母偏好密码子,化学合成了家蝇防御素抗菌肽成熟肽基因(des-hf)。将合成的家蝇防御素抗菌肽成熟肽基因(des-hf)克隆入pPICZα-A载体中构建分泌型重组酵母表达载体pPICZα-A-des-hf,转化Pichia pastoris受体菌SMD1168。在甲醇氧化酶(AOX)启动子调控下,分子量约5kD的des-hf抗菌肽获得表达。初步抑菌活性测定,des-hf抗菌肽对革兰氏阳性菌有很强的抑菌活性。
Insect defensins as a sort of defesins are a family of novel peptides with anti-extrogenous pathogen activities. They are a class of small cationic antibiotic peptides that have abundant of arginine (Arg). Insect defensins selectively kill Gram-positive bacteria. Using the preferential condon of Pichia pastoris, a novel antibacterial peptide Musca domestica Defensin (des-hf) 140bp in length was designed and synthesized according to the sequence from GenBank(AY260152). The modified antibacterial peptide gene was cloned into the pPICZα-A vector to construct the recombinant expression vector pPICZα-A-des-hf and then transformed into yeast host strain SMD1168. Under the control of the promoter AOX1 (alcohol oxidase 1), the protein with a similar molecular weight to native antibacterial peptide was expressed and secreted into the supernatant. Des-hf peptide showed potent antibacterial activity against Staphylococcus aureus in elementary Secretion expression.
仇妍虹. 家蝇防御素抗菌肽基因的密码子优化及在毕赤酵母中的分泌表达[J]. 中国生物工程杂志, 2006, 26(0): 90-94.
. Codon Optimization of Antibacterial Peptide Musca domestica Defensin Gene and expression in Pichia pastoris. China Biotechnology, 2006, 26(0): 90-94.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/90
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