以卡拉胶为诱导物使解淀粉枯草芽孢杆菌进行固体发酵,产生卡拉胶降解酶。通过降解产物粘度的变化来确定适宜的发酵条件及降解条件。结果表明发酵物中的液体种子接种量为2%,卡拉胶的终浓度为0.1%,降解温度为50℃,pH=5,加酶量为0.5:1(w/w,酶:底物)时,此酶的降解效果最好。卡拉胶分子量由最初的1296kDa降至1660Da。
Carrageenase were produced by Bacillus amyloliquefaciens, which fermented with carrageen as inductor. The optimal condition of zymosis and degradation determined by viscosity change was achieved: liquid seed inoculate 2%, carrageen terminal concentration was 0.1% in the solid leavening, and degrading temperature of 50℃, pH 5, the enzyme quantity was 0.5:1(w/w). Its molecular weight came down from 1296kDa to 1660Da.
那晶,张文清,夏玮,刘书琦,李青青. 酶法降解卡拉胶的研究[J]. 中国生物工程杂志, 2008, 28(专刊): 116-119.
. Study on Carrageen Enzymolysis. China Biotechnology, 2008, 28(专刊): 116-119.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I专刊/116
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