基因表达的连续分析技术(serial analysis of gene expression SAGE)是应用于基因研究非常有利的工具,具有高通量、高保真度、既定性又定量等诸多优点。作者经过广泛查阅近年来相关国内外文献, 密切关注肝脏研究领域,并结合自身研究成果, 发现SAGE技术较前有了很大进步。这主要体现在三个方面:SAGE技术本身更趋优化,数个SAGE文库联合分析,结合其它基因研究手段进行研究。SAGE技术的原理、方法进展进行简单综述,以应用于肝脏中的研究为媒介,重点探讨其在应用方面突破。
The serial analysis of gene expression (SAGE) technology is a very useful skill to do the researches on gene. It has many advantages, such as high throughout, high-fidelity, qualitation and quantitation. Based on our own researches and combined with the reviews of the related literature at home and abroad, we analyzed and evaluated the latest development of the research on SAGE, especially the development of principles, methods and applications of the technology. The improvements include three aspects, the optimization of SAGE itself, analysis among several SAGE libraries, combining with other gene researching methods. Through the whole view of SAGE used in liver researches, we find that the SAGE technology has been improved to some degree, especially, it's applications in many fields are greatly extended. The principles of SAGE change little, the methods of SAGE has been improved a lot.
刘卫辉,窦科峰. SAGE技术及其在肝脏研究中的进展[J]. 中国生物工程杂志, 2008, 28(专刊): 183-186.
LIU Wei-Hui Ke-Feng DOU. Latest developments of SAGE technology and it's applications in liver. China Biotechnology, 2008, 28(专刊): 183-186.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I专刊/183
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